High performance thin layer chromatography (HPTLC) combined with on-spot detection and characterization via easy ambient sonic-spray ionization mass spectrometry (EASI-MS) is applied to the analysis of biodiesel (B100) and biodiesel-petrodiesel blends (BX). HPTLC provides chromatographic resolution of major components whereas EASI-MS allows on-spot characterization performed directly on the HPT...

A simple, rapid and reliable HPTLC method has been developed for simultaneous estimation of Chebulagic acid and Chebulinic acid in marketed polyherbal formulations. Identification and quantification were performed on 10cm x 10cm, layer thickness 0.2mm, aluminumbacked silica gel 60 F254 HPTLC plates previously washed with methanol. The solvent system consisting of Ethyl Acetate: Toluene: Formic ...

BACKGROUND & OBJECTIVES Cannabis is one of the most commonly abused drugs worldwide. There is a distinct clinical correlation between cannabis abuse and mental disorders. However, it is essential to establish cannabis intake in the abusers in order to establish causality between cannabis and psychiatric illness. The limitations of current detection methods using commercial cassettes prompted us...

background and objectives: traditional polyherbal oils are still in use in persian traditional medicine (ptm). most of these formulations are prepared via traditional procedures such as maceration of herbs in oils or evaporating aqueous herbal extracts in boiling or heating oils as the vehicle. thus, their quality control, standardization and authentication are real challenges due to the lack o...

The present study investigates a quantitative high performance thin layer chromatographic (HPTLC) method for chlorogenic acid and hyperoside determination from berry extracts. Chlorogenic acid and hyperoside were extracted using a methanol and water mixture and separated on a HPTLC 60F254 silica gel plates with ethyl acetate, acetic acid, formic acid and water 10:1.1:1.1:2.3 (v/v/v/v) as mobile...

The ethyl acetate partition of dried methanolic extract of aerial parts of Euphorbia microsciadia (Euphorbiaceae) afforded three pentacyclic triterpenes, betulinic acid (1) from lupane type, oleanolic acid (2) from oleane type and ursolic acid (3) from ursane type triterpenes that are reported for the first time in this plant. These three compounds were structurally compared through their mass ...

Structure-activity relationships of ursane-type pentacyclic triterpenes obtained from natural sources and by chemical derivatization are reviewed. Ursolic acid, corosolic acid, and a new ursane-type pentacyclic triterpene, 7,24-dihydroxyursolic acid, were isolated from the methanolic extract of the leaves of the Bangladeshi medicinal plant, Saurauja roxburghii. Derivatization of ursolic acid by...

A new naturally occurring compound based on the dammarane skeleton, i.e. cabralealactone 3-acetate-24-methyl ether, was isolated from the aqueous methanolic extract of Forsythia koreana fruits, along with eight known compounds: cabralealactone 3-acetate, ursolic acid, arctigenin, arctiin, phillyrin, rutin, caffeic acid, and rosmarinic acid. The identification of the isolated compounds was based...

Ursolic acid is a very important compound due to its biological potential as an anti-inflammatory, trypanocidal, antirheumatic, antiviral, antioxidant and antitumoral agent. This study presents the HPLC analysis of ursolic acid (UA) content in eight different Ocimum species: O. americanum L., O. basilicum L, O. basilicum var purpurascens Benth, O. basilicum var. minimum L, O. gratissimum L, O. ...