A simple, sensitive and precise RP-HPLC method was developed for the determination of dutasteride in tablet dosage form. The RP-HPLC separation was achieved on phenomenex C(18) column (250 mm, id 4.6 mm, 5 mum) using mobile phase methanol:water (90:10 v/v) at a flow rate of 1 ml/min at an ambient temperature. Quantification was achieved with photodiode array detection at 235 nm over the concent...

Curcumin is the main compound in turmeric and has problems its stability. The analytical method used to determine curcumin levels by using high performance liquid Chromatography (HPLC) Photodiode Array Detector (PDA). HPLC a separation capability good reproducibility with low detection limit. purpose of this research that isolation curcumine determined HPLC-PDA been validated can be applied as ...

A simple and reliable high performance liquid chromatography (HPLC) method has been developed for the simultaneous quantification of five major bioactive components in 'Shu-Jin-Zhi-Tong' capsules (SJZTC), for the purposes of quality control of this commonly prescribed traditional Chinese medicine. Under the optimum conditions, excellent separation was achieved, and the assay was fully validated...

Cercosporin has excellent properties of photosensitization that have been widely used in organophotocatalyst and photodynamic therapy as well an antimicrobial agent. Therefore, there is a need to quantify it accurately with accessible methods. A comparative analysis cercosporin quantification obtained by spectrophotometry (SPEC) high-performance liquid chromatography (HPLC) was performed for ni...

The study of triglycerides gastrointestinal fate is main importance due to their nutritional and health implications. In this matter, there a need for specific, reliable fast method quantify lipid digestion products. HPLC-CAD developed in work proposes manner simultaneously triolein, sn-1,2/2,3-diolein, sn-1,3-diolein, sn-2-monoolein, sn-1/3-monoolein oleic acid. First, the HPLC separation was ...

Purpose: To develop, validate and compare two chromatographic methods - high performance liquid chromatography with diode array detector ((HPLC-DAD) ultraviolet detection (UPLC-UV) for the effective analysis of polyphenols in Moringa oleifera leaves.Methods: HPLC-DAD UPLC-UV were applied accurate determination eleven major leaves. The conditions was determined on C18 column by gradient elution ...

Creatinine is an important biomarker for renal function diagnosis and normalizing variations in urinary drug/metabolites concentration. Quantification of creatinine in biological fluids such as urine and plasma is important for clinical diagnosis as well as in biomonitoring programs and urinary metabolomics/metabonomics research. Current methods for creatinine determination either are nonselect...

BACKGROUND Sequential injection chromatography (SIC) is a new alternative separation technology. It has some advantages over high performance liquid chromatography (HPLC) regarding simplicity, inexpensiveness, portability, ease of use, maintenance requirement and operation time. In contrast, SIC has had suffered from some limitations. RESULTS The current work involves four achievements. (a) O...

A simple, rapid, and highly selective HPLC-DAD method was developed for the simultaneous determination of diclofenac sodium (DIC) and diflunisal (DIF) in pure form and in their combined formulation. Effective chromatographic separation was achieved using a Zorbax SB-C8 (4.6×250 mm, 5 μm particle size) column with a mobile phase composed of 0.05 M phosphoric acid, acetonitrile, and methanol in t...

The high-performance liquid chromatography (HPLC) method with photodiode array (PDA) and fluorescence (FL) detection for the simultaneous separation and determination of caffeine and water-soluble vitamins is described. The method is relatively rapid: 13 compounds can be analyzed within 30 min: caffeine; ascorbic acid (vitamin C); thiamine (vitamin B1); riboflavin 5-phosphate (FMN, flavin monon...