A simple high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) method for screening and quantification of ricinine in feeds is established for use in feed quality control. Ricinine is extracted from the sample with methanol. The sample extract is directly used for screening with LC-MS and is cleaned up using solid-phase extraction for quantification w...

Aflatoxins, zearalenone, deoxynivalenol, fumonisins, and their respective metabolites require specific procedures for their determination because of their diverse chemistry and occurrence in complex matrices of feedstuffs and foods. Major sources of error in the analysis of these mycotoxins arise from inadequate sampling and inefficient extraction and cleanup procedures. The determinative step ...

In the biological sciences, mass spectrometry is one of the most popular methods employed for the determination of the molecular weights and structures of metabolites. The popularity of mass spectrometry is due to the high sensitivity of the analytical method and the possibility of obtaining molecular weights and structure of metabolites directly from high performance liquid chromatography (HPL...

apigenin is best known as an irreversible inhibitor of monoamine oxidase (mao), an intracellular enzyme associated with the outer membrane of mitochondria. the purpose of this study is to establish a reliable and quick method for the assignment of apigenin in cosmos bipinnatus, apium graveolens and petroselinum crispum by high performance liquid chromatography with ultraviolet detection (hplc-u...

Tamoxifen-induced DNA adducts were analyzed with the (32)P-postlabeling method using high-performance liquid chromatography (HPLC)-radioactivity detection from endometrial tissue of breast cancer patients and controls. Liver DNA from tamoxifen-treated rats was used as a positive standard. In blind analysis, five of the seven samples from tamoxifen-treated patients showed DNA adducts; none of th...

This HPLC method for measuring antipyrine, lorazepam, and indocyanine green in 0.5 mL of plasma can be used in studies of liver function in which these "model" compounds are used. After a fast, simple, one-step extraction procedure with acetonitrile, an isocratic HPLC system is used, with a single detection wavelength (214 nm) and a single internal standard (1-acetamidopyrene). The mobile phase...