نتایج جستجو برای: hph selectable gene
تعداد نتایج: 1142821 فیلتر نتایج به سال:
A full length, double-stranded ovalbumin complementary DNA (cDNA) was synthesized in vitro and used to assay the action of 35 restriction endonucleases upon this DNA sequence. Some 22 enzymes, Bal I, BamHl, Blu I and its isoschizomerXho I, Ppu 1,Xba I, Tag I, Sal I, Bgl I, Bgl II, Kpn I, Sst II, Hue II, Hpa I, Hap II, Hha I, Hind11 and its isoschizomer HincII, Hind111 and its isoschizomer Hsu I...
Gene MAGEA1 belongs to a group of human germline-specific genes that rely on DNA methylation for repression in somatic tissues. Many of these genes, termed cancer-germline (CG) genes, become demethylated and activated in a wide variety of tumors, where they encode tumor-specific antigens. The process leading to DNA demethylation of CG genes in tumors remains unclear. Previous data suggested tha...
A new expression cassette (EC0) consisting of the fused 5' and 3' intergenic regions (IGRs) of the Eremothecium cymbalariae translational elongation factor 1α (EcTEF1) gene was evaluated through expression of the bacterial hygromycin B phosphotransferase (hph) resistance gene in the common baker's yeast Saccharomyces cerevisiae. Progressively shorter versions of the hph-containing EC cassette (...
The ability to conveniently construct gene disruptions is an important methodology for genetic analysis of the fission yeast Schizosaccharomyces pombe. Because of the limited number of selectable markers available for generating gene disruptions in fission yeast, the construction of strains that contain multiple gene disruptions can be quite difficult. This becomes a particular problem when epi...
A three-plasmid yeast expression system utilizing the portable small ubiquitin-like modifier (SUMO) vector set combined with the efficient endogenous yeast protease Ulp1 was developed for production of large amounts of soluble functional protein in Saccharomyces cerevisiae. Each vector has a different selectable marker (URA, TRP, or LEU), and the system provides high expression levels of three ...
Variation of transgene expression caused by either position effect at the insertion site or the promoter/enhancer elements employed for the expression of selectable marker genes has complicated phenotype characterization and caused misinterpretation. We have developed a reporter system in rice to analyze the influence of vector configuration, spacer and selectable marker gene promoter on the ex...
(6R)-5,6,7,8-Tetrahydro-biopterin (H(4)B) is essential for the catalytic activity of all NO synthases. The hyperphenylalaninemic mouse mutant (hph-1) displays 90% deficiency of the GTP cyclohydrolase I, the rate-limiting enzyme in H(4)B synthesis. A relative shortage of H(4)B may shift the balance between endothelial NO synthase (eNOS)-catalyzed generation of NO and reactive oxygen species. The...
Infantile hypertrophic pyloric stenosis (IHPS): A study of its pathophysiology utilizing the newborn 1 hph-1 mouse model of the disease 2 3 Running Title: Rho-kinase and infantile pyloric stenosis 27 28 29 30 31 Abstract 33 Infantile hypertrophic pyloric stenosis (IHPS) is a common disease of unknown etiology. The 34 tetrahydrobiopterin (BH4)-deficient hph-1 newborn mouse has a similar phenotyp...
The engineering of novel properties and functions into viral vectors for improved gene delivery remains a barrier to the development of efficient, customized gene delivery vehicles. Rational methods for designing improved viral vectors are often experimentally challenging and laborious, particularly when knowledge of viral structure-function relationships is limited. As an alternative, high-thr...
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