In the pigeon, the nucleus of the basal optic root, a component of the accessory optic system, projects directly upon the vestibulo-cerebellum. This nucleus receives a prominent projection composed of large-diameter retinal axons, known as the basal optic root. The cells of origin of this tract were identified using horseradish peroxidase (donor:hydrogen-peroxide oxidoreductase, EC 1.11.1.7) as...

Cultured bovine adrenal medullary cells are an excellent preparation for quantitative analysis of the secretory exocytosis/endocytosis cycle. In this paper we examine the kinetics of endocytosis after stimulation of secretion. Membrane retrieval was monitored by uptake of the fluid phase marker horseradish peroxidase. Horseradish peroxidase was found to be suitable because it can be washed off ...

A blue-LED-driven two-liquid-phase system has been set up for the in situ activation of horseradish peroxidase avoiding use hydrogen peroxide and drawbacks related to enzyme denaturation undesired radical side-reactions. The photobiocatalytic was applied oxidative coupling natural synthetic coumarins bicoumarins, allowing obtain homodimers only one step, tedious protecting groups. Two C-2 symme...

Two limiting structures may be assigned to the primary derivative of peroxidases (Compound I) demanding either (a) total or (b) partial retention of peroxide components. It is now shown that (a) interaction of horseradish peroxidase with m-nitroperbenzoic acid, in 15 mM phosphate, pH 6.2, 25", leads to the stoichiometric formation of the enzymeperoxide derivative, m-nitrobenzoate and 1 proton; ...

A highly sensitive method for the light microscopic in situ hybridisation of immunoglobulin light chain mRNA in formalin fixed, paraffin wax embedded sections is reported. This method is based on signal amplification using horseradish peroxidase catalysed deposition of biotinylated tyramine at the sites of hybridisation. kappa and lambda light chain immunoglobulin mRNA in situ hybridisation was...

The redox couple between ascorbic acid (vitamin C) oxidation and reduction of the semiquinoid form of p-phenylenediamine (PPDA) is the basis of a new chronometric assay of vitamin C. Commercial horseradish peroxidase or citrus peroxidase isolated from seeds catalyzes the hydroperoxidation of PPDA through a re versible semiquinoid form to a colored condensa tion product. Ascorbic acid interrupts...