Purpose To investigate the effects of homocysteine on choroidal angiogenesis, we established an ex vivo choroidal sprouting explant model and examined the potential growth factors for angiogenesis. Methods Choroid fragments with retinal pigment epithelium were isolated from mouse and embedded in Matrigel. Homocysteine at different concentrations were added to the culture mediums. The choroida...

Rats were fed diets with and without 0.5% L-cysteine supplement for 14 d or shorter periods to clarify the mechanism by which dietary cysteine elicits its hypohomocysteinemic effect. Cysteine supplementation significantly decreased plasma homocysteine concentration with an increase in plasma cysteine concentration in rats fed 10% casein diet (10C) or 15% soybean protein diet (15S) but not in ra...

Although the importance of methyl metabolism in fetal development is well recognized, there is limited information on the dynamics of methionine flow through maternal and fetal tissues and on how this is related to circulating total homocysteine concentrations. Rates of homocysteine remethylation in maternal and fetal tissues on days 11, 19, and 21 of gestation were measured in pregnant rats fe...

Elevated homocysteine levels have been associated with an increased risk of ischemic stroke in various populations. This study aims to find the association between and Pakistani population. The recruited 100 cases controls without any history from two tertiary care hospitals Pakistan. Homocysteine were measured both groups using a chemiluminescent microparticle immunoassay. mean level was signi...

The amino acid, homocysteine, is not supplied by food but is a product formed by cleavage of S-adenosylhomocysteine; a product of transmethylation. Homocysteine is further salvaged to methionine. Since this reaction is in most tissues dependent on 5-methyltetrahydrofolate, we investigated the effect of the antifolate drug, methotrexate (MTX), on homocysteine in patients treated with this drug a...

BACKGROUND Proinflammatory cytokines play key roles in atherogenesis and disease progression. Because hyperhomocysteinemia is an independent risk factor for cardiovascular disease, we hypothesized that homocysteine could be atherogenic by altering the expression of specific cytokines in vascular endothelial cells. METHODS AND RESULTS Northern blot and RNase protection assays showed that DL-ho...

OBJECTIVES To investigate the effect of higher weekly maintenance dose methotrexate (MTX) (> or =25 mg/week) on plasma homocysteine concentrations in adults with RA. METHODS Patients with RA were treated with high doses of MTX with adjuvant folic acid. Plasma homocysteine was determined at baseline and 1, 2, 4, 8, 12, and 48 hours after subcutaneous MTX administration. Maximum homocysteine co...

Normal folate, homocysteine, and methyl group metabolism is critical for maintaining optimum health, and perturbations in these mechanisms may lead to a number of pathological conditions such as diabetes, cardiovascular disease, neural tube defects, and cancer. Hyperhomocysteinemia is a condition that occurs when blood plasma concentrations of homocysteine exceed 15μM and has been implicated as...

BACKGROUND The hippocampus is important for memory. Hippocampal atrophy and higher levels of homocysteine may both predict cognitive dysfunction in community-dwelling older people. We tested if higher homocysteine relates to hippocampal thinning in this group. SUBJECTS 156 community-dwelling volunteers without clinical memory problems. METHOD We measured minimal hippocampal widths on magnet...

OBJECTIVES To compare serum concentrations of homocysteine in healthy dogs and those fitting the criteria for systemic inflammatory response syndrome and to compare these values to commonly measured B-vitamins. METHODS Study dogs were classified into non-infectious systemic inflammatory response syndrome or sepsis groups and blood was drawn on Day 1 of the patient's hospitalisation for measur...