We present here a novel platform combination, using a multifunctional pipette to individually electroporate single-cells and to locally deliver an analyte, while in their culture environment. We demonstrate a method to fabricate low-resistance metallic electrodes into a PDMS pipette, followed by characterization of its effectiveness, benefits and limits in comparison with an external carbon mic...

BLOOD PIPETTE SHAKING MACHINE MARK II BY K. C. PARRY Fromn the Medical Division, Atomic Energy Research Establishment, Harwell (RECEIVED FOR PUBLICATION JUNE 22, 1950) The blood pipette shaking machine previously reported (Parry and Simmonds, 1950) has been modified to incorporate various improvements. In the new machine (1) the pipettes lie in the same plane and there is greater ease of insert...

An unexpected collaboration between a synthetic biology lab at UCSF and a Palo Alto-based design firm is stirring up the way that research is conceived and conducted, by integrating innovation and "design thinking" into the scientific method.

A contact lens may be removed from the eye either manually or by using a small rubber sucker. The removal of lenses by the rubber sucker technique is difficult at times, especially when the lens is fenestrated, as the vacuum cannot be maintained. Assistance in the removal of the lens may be obtained by modifying existing rubber suckers. The bulb is cut off with a sharp blade and into the lumen ...

The quest for nonoptical imaging methods that can surmount light diffraction limits resulted in the development of scanning probe microscopes. However, most of the existing methods are not quite suitable for studying biological samples. The scanning ion conductance microscope (SICM) bridges the gap between the resolution capabilities of atomic force microscope and scanning electron microscope a...

Beta-Adrenoceptor agonists activate a time- and voltage-independent Cl- conductance in mammalian cardiac myocytes. To characterize the cellular signaling pathways underlying its regulation, wide-tipped pipettes fitted with a pipette perfusion device were used to record whole-cell current and to introduce nucleotides to the interior of guinea pig ventricular myocytes. Replacement of pipette GTP ...

The software for analysis of micropipette aspiration images is written in Python using the package wxPython for graphical user interface, PIL for image loading, matplotlib for outputs plotting and SciPy for numerical analysis. Since the studied system, vesicle aspirated in a cylindrical pipette, is axis-symmetric, the exact geometry is defined by a few parameters only and there is no need of fi...

Measuring cellular respiration with single-cell spatial resolution is a significant challenge, even with modern tools and techniques. Here, a double-channel micropipette is proposed and investigated as a probe to achieve this goal by sampling fluid near the point of interest. A finite element model (FEM) of this perfusion probe is validated by comparing simulation results with experimental resu...