Gastrin was given to rats by continuous subcutaneous infusion through implanted osmotic minipumps in doses covering a wide range of the dose response relationship for gastrin with regard to the trophic effect on the enterochromaffin like cells of the oxyntic mucosa. Thirty five rats were divided into five groups (each of seven rats), one group receiving a control solution of 1% albumin, the oth...

Histidine decarboxylase (HDC) from Lactobacillus 30a produces histamine that is essential to counter waste acids, and to optimize cell growth. The HDC trimer is active at low pH and inactive at neutral to alkaline pH. We have solved the X-ray structure of HDC at pH 8 and revealed the novel mechanism of pH regulation. At high pH helix B is unwound, destroying the substrate binding pocket. At aci...

The diverse bacteria encoding histidine decarboxylase gene during the fermentation of Sichuan-style sausages were investigated by culture-dependent techniques, polymerase chain reaction–denaturing gradient gel electrophoresis (PCR-DGGE), and high-throughput sequencing. All microbial indicators exhibited advantages mixed starter culture stability microecosystem was more in inoculation group than...

In studies on the nature of the enzymic defect in patients with histidinaemia, an inborn error of histidine metabolism (La Du, Howell, Jacoby, Seegmiller & Zannoni, 1962), it was necessary to develop a sensitive assay procedure to measure histidine oc-deaminase (L-histidine ammonia-lyase, EC 4.3.1.3), the enzyme that converts histidine into urocanic acid. The application of this method has show...