نتایج جستجو برای: his beloved woman

تعداد نتایج: 243129  

Journal: :Methods in molecular biology 2009
Brian P Austin Sreedevi Nallamsetty David S Waugh

Insolubility of recombinant proteins in Escherichia coli is a major impediment to their production for structural and functional studies. One way to circumvent this problem is to fuse an aggregation-prone protein to a highly soluble partner. E. coli maltose-binding protein (MBP) has emerged as one of the most effective solubilizing agents. In this chapter, we describe how to construct combinato...

Journal: :Journal of bacteriology 2005
Julio Ramos Aires Hiroshi Nikaido

To understand better the mechanisms of resistance-nodulation-division (RND)-type multidrug efflux pumps, we examined the Escherichia coli AcrD pump, whose typical substrates, aminoglycosides, are not expected to diffuse spontaneously across the lipid bilayer. The hexahistidine-tagged AcrD protein was purified and reconstituted into unilamellar proteoliposomes. Its activity was measured by the p...

Journal: :Acta crystallographica. Section D, Biological crystallography 2002
David M Duda Craig Yoshioka Lakshmanan Govindasamy Haiqian An Chingkuang Tu David N Silverman Robert McKenna

Carbonic anhydrases catalyze the interconversion of carbon dioxide to bicarbonate. Human carbonic anhydrase isozyme III with a C-terminal hexahistidine tag was overexpressed in Eschericha coli, purified and crystallized. Diffraction data (93.4% completeness) were collected to 2.2 A resolution on an in-house R-AXIS IV++ image-plate system with Osmic mirrors and a Rigaku HU-H3R CU rotating-anode ...

2016
Alexander Kinna Berend Tolner Enrique Miranda Rota Nigel Titchener‐Hooker Darren Nesbeth Kerry Chester

Fusion-tag affinity chromatography is a key technique in recombinant protein purification. Current methods for protein recovery from mammalian cells are hampered by the need for feed stream clarification. We have developed a method for direct capture using immobilized metal affinity chromatography (IMAC) of hexahistidine (His6) tagged proteins from unclarified mammalian cell feed streams. The p...

Journal: :Journal of molecular recognition : JMR 2009
Steven Knecht Daniel Ricklin Alex N Eberle Beat Ernst

Since immobilized metal ion affinity chromatography (IMAC) was first reported, several modifications have been developed. Among them, Ni(2+) immobilized by chelation with nitrilotriacetic acid (NTA) bound to a solid support has become the most common method for the purification of proteins carrying either a C- or N-terminal histidine (His) tag. Despite its broad application in protein purificat...

Journal: :بحوث فی اللغه العربیه 0
زهرا سلیمان پور دانشگاه فرهنگیان منصوره زرکوب

woman in motran's poem -abstract this article explains the issue of woman in two fields of social and romance. in social case, motran defends the rights of woman and considers her as a human with its rights and one of the two major factors in the society. woman is a basic and essential party in building the social and even political body of the society, at motran's point of view. the ...

Journal: :PMLA/Publications of the Modern Language Association of America 1997

Journal: :Verbum et Ecclesia 1994

Journal: :The Journal of biological chemistry 2003
Joachim Klein Mark Nolden Steven L Sanders Jay Kirchner P Anthony Weil Karsten Melcher

An important goal is to identify the direct activation domain (AD)-interacting components of the transcriptional machinery within the context of native complexes. Toward this end, we first demonstrate that the multisubunit TFIID, SAGA, mediator, and Swi/Snf coactivator complexes from transcriptionally competent whole-cell yeast extracts were all capable of specifically interacting with the prot...

2014
Dale A Shepherd Antonio Ariza Thomas A Edwards John N Barr Nicola J Stonehouse Alison E Ashcroft

RATIONALE Bunyaviruses have become a major threat to both humans and livestock in Europe and the Americas. The nucleocapsid (N) protein of these viruses is key to the replication cycle and knowledge of the N oligomerisation state is central to understanding the viral lifecycle and for development of therapeutic strategies. METHODS Bunyamwera virus and Schmallenberg virus N proteins (BUNV-N an...

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