Irradiation of previously dark-grown cell suspension cultures from parsley (Petroselinum hortense Hoffm.) with ultraviolet light caused large, concomitant increases in mRNA activities for two characteristic enzymes of phenylpropanoid metabolism, phenylalanine ammonia-lyase, and flavanone synthase. The rates of enzyme synthesis both in vitro in a reticulocyte lysate and in vivo were quantitated ...

In recent years, the application of natural substances on crops has been intensified in order to increase the resistance and yield of the soybean crop. Among these products are included plant biostimulants that may contain algae extracts, amino acids, and plant regulators in their composition. However, there is little information on the isolated effect of each of these constituents. The objecti...

Acrylic acids and alanines substituted with heteroaryl groups at the beta-position were synthesized and spectroscopically characterized (UV, HRMS, (1)H NMR, and (13)C NMR spectroscopy). The heteroaryl groups were furanyl, thiophenyl, benzofuranyl, and benzothiophenyl and contained the alanyl side chains either at the 2- or 3-positions. While the former are good substrates for phenylalanine ammo...

An experiment was conducted to identify the biochemical defense enzymes, viz. catalase (CAT), peroxidase (POX), phenylalanine ammonia lyase (PAL) and total phenol responsible for resistance against A. solani in resistant (cv. H-88-78-1) susceptible (Punjab Chhuhara) cultivars of tomato after application Amistar 25 SC (azoxystrobin 0.1%) during 2015 crop season. Azoxystrobin applied their effica...

Early blight of tomato (Solanum lycopersicon L.) incited by Alternaria solani is highly destructive disease in the world. Environmental factors significantly impact early epidemics, leading to loss up 78 per cent production. Twenty genotypes were used this study identify resistant and susceptible selected represent a range reactions when screened under field conditions. The plants evaluated for...

Previously, we found that phenolic content and antioxidant capacity (AOX) in carrots increased with wounding intensity. It was also reported that UV radiation may trigger the phenylpropanoid metabolism in plant tissues. Here, we determined the combined effect of wounding intensity and UV radiation on phenolic compounds, AOX, and the phenylalanine ammonia-lyase (PAL) activity of carrots. Accordi...

Pharmacological evidence implicates trans-cinnamic acid as a feedback modulator of the expression and enzymatic activity of the first enzyme in the phenylpropanoid pathway, L-phenylalanine ammonia-lyase (PAL). To test this hypothesis independently of methods that utilize potentially non-specific inhibitors, we generated transgenic tobacco lines with altered activity levels of the second enzyme ...

The study focuses on the effect of supplemental UV-B radiation on germination, seedling growth, chlorophyll a and b contents, soluble phenols, anthocyanins, flavones contents, phenylalanine ammonia lyase (PAL) activity and tyrosine ammonia lyase (TAL) activity of mash-bean (Vigna mungo (L.) Hepper.). Even though the germination velocity was substantially increased, the final germination percent...

In this study it was aimed to investigate inducibility of secondary metabolism in Astragalus chrysochlorus by yeast extract which is known to cause the synthesis of defensive phenolic metabolites. Cell suspension cultures of Astragalus chrysochlorus responded to elicitor treatment (10 gl(-1) yeast extract) by increasing phenylalanine ammonia lyase (PAL E.C. 4.3.1.5) activity the key enzyme of p...

Phenylalanine Ammonia-Lyase, Protein Metabolism, Regulation, /ra/js-Cinnamic Acid, Daucus carota, Suspension Culture In vivo and in vitro experiments were performed in order to study the regulatory role of transcinnamic acid and its hydroxylated derivatives (p-coumaric acid, caffeic acid) on the deamina­ tion of phenylalanine catalyzed by PAL (EC 4.3.1.5). 7>arts-cinnamic acid inhibits growth a...