High performance thin layer chromatographic finger print parameters had been developed for the siddha medicine 'Maka Vallati Ilekiyam' to fix standards.

The first atropisomeric phosphinine was designed and prepared by introducing substituents into specific positions of the heterocyclic framework; the presence of axial chirality was predicted by means of DFT calculations and experimentally verified by chiral HPLC analysis, derivatization experiments as well as temperature dependent 31P{1H} NMR spectroscopy.

ABSTRACT The retention behaviour of semicarbazides and their cyclic analogues 1,2,4-triazol-3-ones, has been investigated by RP-8, RP-18 and IAM HPLC. The structures of new derivatives were proved by elemental analyses, IR, 1H NMR and 13C NMR spectroscopy. The compounds showed regular retention behaviour in three chromatographic systems; their log k values decreased linearly with the increasing...

Rapid microchip reversed-phase HPLC of peptides and proteins at pressure gradients of 12 bar/cm (180 psi/cm) has been performed using a microdevice that integrates subnanoliter on-chip injection and separation with a miniaturized fluorescence detector. Proteins and peptides were separated on a C18 side-chain porous polymer monolith defined by contact lithography, and injection was achieved via ...

A simple, sensitive isocratic method for the detection and quantification of ciprofloxacin in plasma and urine has been developed. The assay consisted of reversed-phase HPLC with ultraviolet detection. Plasma proteins were removed by a fast and efficient procedure. For the urine samples, the only required sample preparation was dilution. Separation was achieved on a C18 reversed-phase column. T...

The trifluoromethylation of a higher fullerene mixture followed by HPLC separation allowed the isolation and X-ray structural characterization of C1-C88(CF3)18 and D2-C92(CF3)16 compounds that turned out to be the derivatives of C2-C88 (isomer 33) and D2-C92 (isomer 82), respectively.

We show herein that establishing a competition between a static receptor and components of dynamic combinatorial libraries to bind an appropriately selected template can be used to determine association constants by HPLC analysis after freezing of the equilibrium.