The effect of phytase on the performance, AMEn, and the ileal digestibility of N and amino acids was investigated in a 15-d trial using day-old male broilers with diets that were low in Ca (0.9% for control and 0.79% for phytase treatment) and available P (AP; 0.45% for control and 0.35% for phytase treatment). The assayed dietary phytase activity of crumble diet was 1,149 phytase unit (FTU)/kg...

A. niger XP isolated from Vietnam produces very low amount of acidic phytase with optimal pH at 2.5 and 5.5. The phytase production of this strain was successfully improved through gene cloning and expression. A 1.4 kb DNA fragment containing the coding region of the phyA gene was amplified by PCR and inserted into the expression vector pPICZαA with a signal peptide αfactor, under the control o...

Phytase, an enzyme that catalyzes the hydrolysis of phytate, was purified from Klebsiella pneumoniae 9-3B. The isolate was preferentially selected in a medium which contains phytate as a sole carbon and phosphate source. Phytic acid was utilized for growth and consequently stimulated phytase production. Phytase production was detected throughout growth and the highest phytase production was obs...

Phytase improves the bioavailability of phytate phosphorus in plant foods to humans and animals and reduces phosphorus pollution of animal waste. Our objectives were to express an Aspergillus niger phytase gene (phyA) in Saccharomyces cerevisiae and to determine the effects of glycosylation on the phytase's activity and thermostability. A 1.4-kb DNA fragment containing the coding region of the ...

Animal nutrition industry demands for phytases with high specific activity and stability under high temperature conditions (feed pellet production) and under acidic conditions (monogastric animals), for this reason continues to stimulate the search for new enzyme sources [1]. Most of scientific work has been focused on Aspergillus spp. phytases due to their biochemical properties found [2]. An ...

A study was conducted to evaluate the ability of the young (0 to 3 wk) broiler chicken to utilize the P provided by a high available P corn [HAPC; 0.27% total P and 0.17% nonphytate P] in comparison with yellow dent corn (YDC; 0.23% total P and 0.03% nonphytate P), and to determine the extent to which supplementation with exogenous phytase enzyme could reduce the demands for dietary P and subse...

The overall objective of the studies reported here was to evaluate the growth and nutrient utilization responses of pigs to dietary supplementation of phytate- or nonstarch polysaccharide-degrading enzymes. In Exp. 1, growth performance and nutrient digestibility responses of forty-eight 10-kg pigs to dietary supplementation of phytase or a cocktail of xylanase, amylase, and protease (XAP) alon...

Low iron and high phytic acid content make fonio based meals a poor source of bioavailable iron. Phytic acid degradation in fonio porridge using whole grain cereals as phytase source and effect on iron bioavailability when added to iron fortified fonio meals were investigated. Grains, nuts and seeds collected in Mali markets were screened for phytic acid and phytase activity. We performed an ir...

A neutral phytase from germinating mung bean (Vigna radiata) seeds dephosphorylates myo-inositol hexakisphosphate sequentially to myo-inositol. The enzyme also binds with higher affinity to myo-inositol trisphosphates (1,4,5), (2,4,5), and (1,3,4) isomers without catalysis. The high affinity complex elicits Ca(2+) mobilization in vitro from microsomes/vacuoles via the formation of a ternary com...

Crossbred pigs (n = 96, BW = 7.5 kg) were used in a 5-wk trial to determine the effectiveness of supplemental Natuphos phytase in improving the bioavailabilities of P and other nutrients in a semipurified diet with soybean meal as the only P source in the basal diet. Two available P (aP) levels (.05 and .16%) and five phytase levels (0, 350, 700, 1,050, and 1,400 units/kg of diet) were used in ...