High density plating procedure was used to evaluate the effect of atrazine on anterior pituitary cells of rats in monolayer culture. Collagenase-dispersed pituitary cells plated in suspension with medium-199 and 10% foetal calf serum attached quantitatively to plastic surfaces within 24 hours. Electron microscopy showed subpopulations of different cell types. After prolonged cultivation, most c...

Animal cell cultures are widely employed for the production of viral vaccines and for recombinant protein expression. The cell line Vero is a continuous, adherent cell line, which has been recommended by the World Health Organization for the production of human vaccines. For the large-scale production of vaccines, microcarriers, which are microspheres that serve as support for the cells, are be...

A multi-wavelength based optical density sensor unit was designed, developed, and evaluated to monitor microalgae growth in real time. The system consisted of five main components including: (1) laser diode modules as light sources; (2) photodiodes as detectors; (3) driver circuit; (4) flow cell; and (5) sensor housing temperature controller. The sensor unit was designed to be integrated into a...

To assess the effect of non chemical management of weed control on forage Sorghum (Sorghum bicolor) a field study was conducted in Varamin, Iran during 2010 crop year in a three- replicated- split factorial experiment laid out in randomized complete block design with four weeding levels (W1= one time cultivation at 3-leaf stage using a duck foot cultivator, W2<...

To assess the effect of non chemical management of weed control on forage Sorghum (Sorghum bicolor) a field study was conducted in Varamin, Iran during 2010 crop year in a three- replicated- split factorial experiment laid out in randomized complete block design with four weeding levels (W1= one time cultivation at 3-leaf stage using a duck foot cultivator, W2<...

To assess the effect of non chemical management of weed control on forage Sorghum (Sorghum bicolor) a field study was conducted in Varamin, Iran during 2010 crop year in a three- replicated- split factorial experiment laid out in randomized complete block design with four weeding levels (W1= one time cultivation at 3-leaf stage using a duck foot cultivator, W2<...

The establishment and cultivation o ľ two human neoplastic cell lines is described. The cell line B-5GT was derived from bono giant cell tumor and B-6F8 from poorly differentiated fibrosarcoma. In comparison to the normal skin fibroblasts both cell lines have a potential for "indefinite" multiplication in vitro and 1 hoy exhibit growth properties which are asso­ ciated with malignant transforma...

Previously, we demonstrated a high quality of minerals formed by serum-free cultured jaw periosteal cells (JPCs) by Raman spectroscopy but the mineralization extent was not satisfactory. In the present study, we analyzed the proliferation and mineralization potential of human platelet lysate- (hPL-) cultured JPCs in comparison to that of FCS-cultured JPCs. By cell impedance measurements, we det...