A differential demand for copper (Cu) of essential cupro-proteins that act within the mitochondrial and chloroplastal electronic transport chains occurs along the daily light/dark cycles. This requires a fine-tuned spatiotemporal regulation of Cu delivery, becoming especially relevant under non-optimal growth conditions. When scarce, Cu is imported through plasma membrane-bound high affinity Cu...

oocyte]. Oocytes were incubated for 24 to 48 hours, treated with coelenterazine [10 ,uM coelenterazine (Molecular Probes, Eugene, OR) and 30 p.M reduced glutathione in OR-2 media (no calcium)] for 2 to 3 hours with gentle orbital shaking in the dark at 18°C, and retumed to ND-86 medium with calcium (maintained in the dark with shaking) until measurements were initiated. For luminometer measurem...

Binding of 3H-folate in Triton X-100 solubilized human prostate homogenate was of a high-affinity type and displayed apparent positive cooperativity typical of specific folate binding. Radioligand dissociation was slow at pH 7.4, but rapid at pH 3.5. Gel chromatography revealed two major folate binding proteins (M(r) approximately 100 and 25 kDa), but only one single band (M(r) approximately 65...

Neuropeptide Y (NPY) is one of the most abundant peptide transmitters in the mammalian brain. In the periphery it is costored and coreleased with norepinephrine from sympathetic nerve terminals. However, the physiological functions of this peptide remain unclear because of the absence of specific high-affinity receptor antagonists. Three potent NPY receptor antagonists were synthesized and test...

The effect of the relative affinity (Ka) on the antitumor efficacy of monoclonal antibodies (MAbs) has been questioned. It has previously been shown in experimental models that the use of MAbs with higher relative Kas manifests itself in a higher percentage of injected dose of MAb bound to tumor. On the other hand, mathematical models have proposed that the use of higher affinity MAbs may be di...

Drug discovery is a difficult and time-consuming process that involves the identification of potent therapeutic | TECH VI EW I agents with good bioavailability, metabolic stability, and low toxicity. An important first step in this process is the identification of molecules that bind with high affinity and selectivity to the biological target. High-affinity ligands might be discovered by screen...

One simple structural change in compound (I), the destruction of its symmetry by its conversion into n-propyl-2-pyridyl disulphide [III, R = C3H7(propyl)-S-S-2-Py], has permitted the demonstration of an important property of the thiol proteinases, the existence of nucleophilic character in the uninterrupted thiol-imidazole interactive systems of their active centres. The reactions of 2-Py-S-S-2...

In-vitro experiments using 203Pb were performed to identify lead-binding components in human haemoglobin. Sephadex A-50 ion-exchange chromatography of haemolysate showed that different types of haemoglobin had different affinities for lead. For the haemolysate from adults, lead was present in both Hb A (alpha 2 beta 2) and Hb A2 (alpha 2 delta 2), whereas, in the haemolysate from new-born infan...