Hemagglutination and fluorescent antibody (FA) are compared for the direct detection of virus devoid of host cells. A determination was made of the minimal number of tissue plaque-forming units of Venezuelan equine encephalomyelitis virus that could be detected by the hemagglutination technique. Similar concentrations of the virus in bovine albumin borate saline, Brain Heart Infusion broth (Dif...

BACKGROUND AND PURPOSE Molecular mimicry was found in the case of Citrobacter braakii O37, which shares epitopes with human erythrocytes. It is believed that erythrocyte-membrane proteins band 3 and glycophorin A (GPA) have common epitopes. Band 3 was recognized by the anti-C. braakii O37 lipopolysaccharide antibodies (LPS-Abs) purified on LPS-affinity columns. This study aimed to investigate t...

The immunosuppressive effects of maintenance combination chemotherapy given for periods ranging from 8 to 28 months to 20 children with acute lymphocytic leukemia in remission were investigated. There was depression of both the primary antibody production to the hemagglutinin antigen of the Hong Kong influenza virus and the anamnestic response to the neuraminidase of the same virus. The primary...

The hemagglutination inhibition test is commonly used for the identification of newly isolated influenza viruses. In this laboratory several difficulties frequently were encountered in typing these new strains after primary isolation by means of this technique. These drawbacks were: (1) Some newly isolated strains are susceptible to nonspecific inhibitors of hemagglutination (Francis, Salk, and...

an indirect enzyme–linked immunosorbent assay (elisa) was developed for screening of antibody to avian infectious bronchitis virus (ibv). antigen was prepared from whole-purified ibv massachusetts serotype (br 801 strain). optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed elisa was determined 0.1μg/ml, 1:3000 and 1:100, respe...

Single radial diffusion (SRD) tests for antibodies to influenza type A hemagglutinin, neuraminidase, nucleoprotein, and matrix protein antigens were compared with conventional hemagglutination inhibition, neuraminidase inhibition, and complement fixation tests. Sera used in this study were obtained in 1968-1969 from volunteers before and after vaccination and before and after an ensuing epidemi...

• Because of evidence relating disturbances in lipid metabolism and transport to atherosclerosis, it seemed of interest to investigate the behavior of sera from affected subjects with a serologically active lipid. This interaction might be reflected in alterations in the serum and thus permit detection and quantitation of the disturbance. Vaccinial hemagglutinin (VHA) appeared to be a likely ma...