DNA fragments encoding the light chain and heavy chain genes of an anti-human HER II antibody, trastuzumab, fused with an egg-lysozyme signal peptide were synthesized based on the codon bias of the methylotrophic yeast Pichia pastoris. These fragments were inserted into a site between the AOX 1-promoter and -terminator in pPICZ A to be expressed by P. pastoris. The expression vector was lineari...

The motor protein kinesin is a tetramer consisting of two heavy and two light chains. Expression of an antisense RNA fragment derived from the mouse ubiquitous kinesin heavy chain (uKHC) cDNA is associated with a unique type of multidrug resistance. We analyzed the effects of retroviral transduction of the human uKHC and its derivatives on drug sensitivity of the human fibrosarcoma cell line HT...

Production of IgG-form bispecific antibody (BsAb-IgG) by co-expressing two antibodies in transfected cells is often inefficient owing to the unwanted pairing between the component heavy and light chains. We have developed an efficient method for the production of a novel IgG-like BsAb by using the natural dimerization mechanism between IgG heavy and light chains. Two single-chain Fv (scFv) of d...

Melem tetramer, the newly synthesized carbon nitride compound is composed of oligomers with four melem units, stable in air, and has improved optical properties compared to melon.

A series of iron complexes bearing a bis(imino)pyridine ligand were synthesised and examined as precatalysts for homopolymerisation of propylene. The alkyl substituents attached to the aryl group on the imine nitrogen atoms significantly affected the catalytic activity and molecular weight of the obtained polypropylenes. Copolymerisation of propylene and various allyl comonomers catalysed by ir...

The steam cracker unit at ORLEN Unipetrol is a significant European producer of monomers. Due to heavy feedstocks, the can produce (besides ethylene, propylene and benzene) very valuable hydrocarbons such as dicyclopentadiene. staff (formerly Chemopetrol) has been investigating possibility producing dicyclopentadiene for almost 40 years. As result many years research activities several generati...

The bacterial metabolism of propylene proceeds by epoxidation to epoxypropane followed by a sequence of three reactions resulting in epoxide ring opening and carboxylation to form acetoacetate. Coenzyme M (2-mercaptoethanesulfonic acid) (CoM) plays a central role in epoxide carboxylation by serving as the nucleophile for epoxide ring opening and the carrier of the C(3) unit that is ultimately c...

The adsorption of propylene on clean and hydrogen-covered Pd(1 1 1) has been studied by using temperatureprogrammed desorption (TPD) and reflection-absorption infrared spectroscopy. Propylene adsorbs in a di-r configuration on clean Pd(1 1 1). At high exposures, it forms second and subsequent layers. Upon heating, propylene desorbs molecularly at 200 and 280 K and, in contrast to ethylene adsor...

Major histocompatibility complex (MHC) class I tetramer technology has become the central technique for analyzing antigen-specific CD8(+) T cell responses and it has been widely used to explore the differentiation and formation of memory CD8(+) T cells. Previously, a simplified and efficient procedure for preparing high quality HLA-A*0201 tetramers has been established in our lab and the tetram...