نتایج جستجو برای: hcv genome

تعداد نتایج: 249841  

Journal: :Journal of clinical microbiology 1997
D Kanistanon M Neelamek T Dharakul S Songsivilai

The genotypic distribution of hepatitis C virus (HCV) isolated from blood donors from four major regions of Thailand was studied by reverse hybridization assays. PCR-amplified products from the 5' noncoding and core regions of the viral genome were hybridized to genotype- and subtype-specific probes which were immobilized on the nitrocellulose membrane. Of 332 anti-HCV-positive plasma samples s...

Journal: :Journal of virology 2015
Yi-Ping Li Santseharay Ramirez Lotte Mikkelsen Jens Bukh

UNLABELLED The first discovered and sequenced hepatitis C virus (HCV) genome and the first in vivo infectious HCV clones originated from the HCV prototype strains HCV-1 and H77, respectively, both widely used in research of this important human pathogen. In the present study, we developed efficient infectious cell culture systems for these genotype 1a strains by using the HCV-1/SF9_A and H77C i...

Journal: :The Journal of general virology 1995
H Tokita H Okamoto P Luengrojanakul K Vareesangthip T Chainuvati H Iizuka F Tsuda Y Miyakawa M Mayumi

Nine (10%) out of 90 hepatitis C virus (HCV) isolates from hepatitis patients and commercial blood donors in Thailand were not classifiable into any of genotypes I/1a, II/1b, III/2a, IV/2b, V/3a or VI/3b by RT-PCR with type-specific primers deduced from the HCV core gene. These isolates were sequenced over a 1.6 kb stretch of the 5'-terminal sequence and 1.1 kb of the 3'-terminal sequence cover...

Journal: :Evolutionary Bioinformatics Online 2008
R.S. Ross J. Verbeeck S. Viazov P. Lemey M. Van Ranst M. Roggendorf

The genome of the hepatitis C virus (HCV) exhibits a high genetic variability. This remarkable heterogeneity is mainly attributed to the gradual accumulation of mutational changes, whereas the contribution of recombination events to the evolution of HCV remains controversial so far. While performing phylogenetic analyses including a large number of sequences deposited in the GenBank, we encount...

Journal: :The Journal of general virology 1993
P Simmonds E C Holmes T A Cha S W Chan F McOmish B Irvine E Beall P L Yap J Kolberg M S Urdea

Hepatitis C virus (HCV) showed substantial nucleotide sequence diversity distributed throughout the viral genome, with many variants showing only 68 to 79% overall sequence similarity to one another. Phylogenetic analysis of nucleotide sequences derived from part of the gene encoding a non-structural protein (NS-5) has provided evidence for six major genotypes of HCV amongst a worldwide collect...

Journal: :Gastroenterology 2011
Mayura M Desai Bin Gong Tehsheng Chan Robert A Davey Lynn Soong Andrey A Kolokoltsov Jiaren Sun

BACKGROUND & AIMS The hepatitis C virus (HCV) serine protease NS3/4A can cleave mitochondria-associated antiviral signaling protein (MAVS) and block retinoic acid-inducible gene I-mediated interferon (IFN) responses. Although this mechanism is thought to have an important role in HCV-mediated innate immunosuppression, its significance in viral persistence is not clear. METHODS We generated tr...

Journal: :The EMBO Journal 2008
Jura Inga Henke Dagmar Goergen Junfeng Zheng Yutong Song Christian G Schüttler Carmen Fehr Christiane Jünemann Michael Niepmann

Hepatitis C virus (HCV) is a positive strand RNA virus that propagates primarily in the liver. We show here that the liver-specific microRNA-122 (miR-122), a member of a class of small cellular RNAs that mediate post-transcriptional gene regulation usually by repressing the translation of mRNAs through interaction with their 3'-untranslated regions (UTRs), stimulates the translation of HCV. Seq...

Journal: :Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases 2009
J Fung C-L Lai M-F Yuen

Chronic hepatitis B virus (HBV) and hepatitis C virus (HCV) infection are the most important causes of hepatocellular carcinoma (HCC), accounting for the majority of the cases worldwide. The geographical distribution of HCC therefore coincides with the distribution of HBV and HCV infections in those areas. Similar to nonviral liver diseases, HBV and HCV infection can cause chronic injury to the...

Journal: :Clinical chemistry 1992
M Saito A Hasegawa T Kashiwakuma M Kohara M Sugi K Miki T Yamamoto H Mori Y Ohta E Tanaka

We developed an enzyme-linked immunosorbent assay (ELISA) system for antibodies to the hepatitis C virus (HCV), using two new recombinant antigens (c11 and c7) derived from the HCV genome. The performance of this ELISA system (Imucheck HCV Ab) was examined. The CV values for both intra-assay precision and reproducibility of identifying HCV antibody in the panel sera ranged from 3.5% to 6.4%. Th...

Journal: :Journal of virology 2003
Edward M Murray Jay A Grobler Eric J Markel Marco F Pagnoni Giacomo Paonessa Adam J Simon Osvaldo A Flores

Progress toward development of better therapies for the treatment of hepatitis C virus (HCV) infection has been hampered by poor understanding of HCV biology and the lack of biological assays suitable for drug screening. Here we describe a powerful HCV replication system that employs HCV replicons expressing the beta-lactamase reporter (bla replicons) and subpopulations of Huh7 cells that are m...

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