Formaldehyde (FA) is a very toxic pollutant. FA in indoor air has a negative effect on human health and should be removed by ventilation or by conversion to non-toxic products. The formaldehyde dehydrogenase (FdDH), a NADand glutathione-dependent enzyme from recombinant methylotrophic yeast Hansenula polymorpha, was tested for its ability to oxidize airborne FA. A continuous fluidized bed biore...

Slodki, M. E. (U. S. Department of Agriculture, Peoria, Ill.), Lynferd J. Wickerham, and M. C. Cadmus. Phylogeny of phosphomannan-producing yeasts. II. Phosphomannan properties and taxonomic relationships. J. Bacteriol. 82:269-274. 1961.-Primitive species of yeasts belonging to the genus Hansenula and closely related genera produce extracellular phosphorylated mannans from glucose. These polysa...

Emp24 is a member of the p24 protein family, which was initially localized to the endoplasmic reticulum, Golgi and COP vesicles, but has recently shown to be associated with Saccharomyces cerevisiae peroxisomes as well. Using cell fractionation and electron- and fluorescence microscopy, we show that in the yeast Hansenula polymorpha, Emp24 also associates with peroxisomes. In addition, we show ...

Cacao beans must be subjected to fermentation before they are used in making chocolate, and their commercial value is related to a proper procedure. Saccharomyces rosei, Hansenula anomala, Pichia fermentans, Pichia membranaefaciens, and Trichosporon cutaneum were found in fermenting cacao beans. All species isolated during the investigation grew on cacao pulp, but only S. rosei, H. anomala, and...

The myxobacterial fungicide, ambruticin, kills the yeast, Hansenula anomala, with high efficacy (MIC 0.05 microg/ml), but only when the cells are growing. The earliest effect, observed almost immediately after the addition of the antibiotic, is a transient but substantial increase of intracellular glycerol, followed by an accumulation of triacylglycerols and free fatty acids. At about the time ...

We show that the synthesis of the N-terminal 50 amino acids of Pex3p (Pex3p(1-50)) in Hansenula polymorpha pex3 cells is associated with the formation of vesicular membrane structures. Biochemical and ultrastructural findings suggest that the nuclear membrane is the donor membrane compartment of these vesicles. These structures also contain Pex14p and can develop into functional peroxisomes aft...

The marked sensitivity of the yeast Hansenula wingei NRRL Y-2340 strain 5 to a new antineoplastic alkaloid served as the basis for a logarithmic-ratio microbiological assay which has been developed for the estimation of concentrations of camptothecin lactone (or cytotoxic equivalents) in tissues of mice. The utility of this assay has been demonstrated by presenting data which show the concentra...

The yeast Williopsis mrakii produces a mycocin or yeast killer toxin designated HMK; this toxin exhibits high thermal stability, high pH stability, and a broad spectrum of activity against other yeasts. We describe construction of a synthetic gene for mycocin HMK and heterologous expression of this toxin in Aspergillus niger. Mycocin HMK was fused to a glucoamylase protein carrier, which result...