نتایج جستجو برای: haloalkane dehalogenase

تعداد نتایج: 829  

Journal: :Biochemistry 1999
J Newman T S Peat R Richard L Kan P E Swanson J A Affholter I H Holmes J F Schindler C J Unkefer T C Terwilliger

The hydrolytic haloalkane dehalogenases are promising bioremediation and biocatalytic agents. Two general classes of dehalogenases have been reported from Xanthobacter and Rhodococcus. While these enzymes share 30% amino acid sequence identity, they have significantly different substrate specificities and halide-binding properties. We report the 1.5 A resolution crystal structure of the Rhodoco...

Journal: :Acta crystallographica. Section D, Biological crystallography 2010
A Stsiapanava J Dohnalek J A Gavira M Kuty T Koudelakova J Damborsky I Kuta Smatanova

The haloalkane dehalogenase DhaA from Rhodococcus rhodochrous NCIMB 13064 is a bacterial enzyme that shows catalytic activity for the hydrolytic degradation of the highly toxic industrial pollutant 1,2,3-trichloropropane (TCP). Mutagenesis focused on the access tunnels of DhaA produced protein variants with significantly improved activity towards TCP. Three mutants of DhaA named DhaA04 (C176Y),...

Journal: :Applied and environmental microbiology 2016
Jacob E Munro Elissa F Liew Mai-Anh Ly Nicholas V Coleman

UNLABELLED 1,2-Dichloroethane (DCA) is a problematic xenobiotic groundwater pollutant. Bacteria are capable of biodegrading DCA, but the evolution of such bacteria is not well understood. In particular, the mechanisms by which bacteria acquire the key dehalogenase genes dhlA and dhlB have not been well defined. In this study, the genomic context of dhlA and dhlB was determined in three aerobic ...

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