Functional screening of an Arabidopsis cDNA library enabled the identification of a novel cDNA, ESR1 (for Enhancer of Shoot Regeneration), that can confer cytokinin-independent shoot formation when overexpressed in Arabidopsis root explants. Neither callus induction nor root formation was affected by ESR1 overexpression. ESR1 encodes a putative transcription factor with an AP2/EREBP domain. Sur...

The CLAVATA (CLV1 and CLV3) and SHOOT MERISTEMLESS (STM) genes specifically regulate shoot meristem development in Arabidopsis. CLV and STH appear to have opposite functions: c1v1 and Clv3 mutants accumulate excess undifferentiated cells in the shoot and floral meristem, while stm mutants fail to form the undifferentiated cells of the shoot meristem during embryonic development. We have identif...

This research was conducted to evaluate the effect of soil application of nitrogen (N) and iron (Fe) fertilizers on morphological traits and shoot mineral contents of bread wheat via factorial arrangement based on randomized complete block design with four replications. The factors included three level of nitrogen (100, 200 and 400 mg N kg-1 dry soil applied) as Ca(NO3)2 and three rate of Iron ...

In order to select the best tomato cultivar for Agrobacterium mediated gene transformation studies, In vitro regeneration frequency of hypocotyls, leaf disc and shoot tip of five tomato cultivars (Lycopersicon esculentum) was investigated on a regeneration medium supplemented with 1 mg/l zeatin and 0.1 mg/l indole-3-acetic acid. Significant differences in regeneration capacity between genotypes...

A factorial completely randomized block design experiment with three replications was carried out in greenhouse to evaluate cereal genotypic variation in phosphorus (P) acquisition and utilization efficiency in a calcareous soil with low available P (4.6 mg P kg-1 soil) and high total P (1260 mg P kg-1 soil). Treatments consisted of eight bread wheat (Triticum aestivum L.), three durum wheat (T...

BACKGROUND AND AIMS Plant architecture and its interaction with agronomic practices and environmental constraints are determinants of the structure of the canopy, which is involved in carbon acquisition and fruit quality development. A framework for the quantitative analysis of grapevine (Vitis vinifera) shoot architecture, based on a set of topological and geometrical parameters, was developed...

The present study was conducted to investigate the effect of growth regulator NAA on in vitro shoot proliferation, rooting, and plantlet establishment. Among the different concentrations of NAA, the best increase in shoot weight (0.25 g) and shoot number (8.83) were observed from 0.1 mg/I NAA. The highest shoot length (2.60 cm), number of leaves (4.83), number of roots (5.15), and root length (...

This work develops methods to account for shoot structure in models of coniferous canopy radiative transfer. Shoot structure, as it varies along the light gradient inside canopy, affects the efficiency of light interception per unit needle area, foliage biomass, or foliage nitrogen. The clumping of needles in the shoot volume also causes a notable amount of multiple scattering of light within c...

Development in higher plants is characterized by the reiterative formation of lateral organs from the flanks of shoot apical meristems. Because organs are produced continuously throughout the life cycle, the shoot apical meristem must maintain a pluripotent stem cell population. These two tasks are accomplished within separate functional domains of the apical meristem. These functional domains ...

A rapid and efficient protocol was developed for inducing direct organogenesis using shoot tip explants of Vernonia cinerea L. Shoot tip explants were cultured on MS medium supplemented with different concentrations of BAP (2.22 to 22.20 μM/l) and KN (2.42 to 23.20 μM/l) for direct shoot induction. The frequency of multiple shoot induction and proliferation increased with increasing concentrati...