Recent transposon mutagenesis studies with two enterohemorrhagic Escherichia coli (EHEC) strains, a sero- type O26:H- strain and a serotype O157:H7 strain, led to identification of a putative fimbrial operon that promotes colonization of young calves (1 to 2 weeks old). The distribution of the gene encoding the major fimbrial subunit present in O-island 61 of EHEC O157:H7 in a characterized set...

A study was done to characterize the shedding of foodborne pathogenic bacteria by Caenorhabditis elegans, evaluate the persistence of worm populations cocultured with foodborne pathogens, and determine if C. elegans disperses ingested pathogens in soil as a result of shedding. Escherichia. coli O157:H7, Salmonella enterica serotype Poona, and Listeria monocytogenes, as well as E. coli OP50, a n...

diarrhea is an important hygienic problem in under development countries. among pathogenic agents escherichia coli is the most etiologic agent of diarrhea in children below five years old. some e. coli strains produce cytotoxins that have cytopathic effect on vero cell and identified as verotoxin. this study performs for resolve frequency of vero toxigenic escherichia coli (vtec) in kerman city...

Twenty-seven Shiga toxin-producing Escherichia coli (STEC) strains were isolated from 207 stx-positive French environmental samples. Ten of these strains were positive for stx(1), and 24 were positive for stx(2) (10 were positive for stx(2vh-a) or stx(2vh-b), 19 were positive for stx(2d), and 15 were positive for stx(2e)). One strain belonged to serotype O157:H7, and the others belonged to sero...

STEC strains can infect extra-intestinal sites such as the human urinary tract and sometimes cause severe complications. We report two cases of urinary tract infection caused by STEC in two elderly women with comorbidities. Although both strains belonged to the O157:H7 serotype and carried genes associated with severe illness, none of the patients developed hemolytic uremic syndrome (HUS). Thes...

Escherichia coli strains that produce Shiga toxins/verotoxins are rare, but important, causes of human disease. They are responsible for a spectrum of illnesses that range from the asymptomatic to the life-threatening hemolytic-uremic syndrome; diseases caused by E. coli belonging to serotype O157:H7 are exceptionally severe. Each illness has a fairly predictable trajectory, and good clinical p...

Strains of enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 produce under stress copious amounts of exopolysaccharide (EPS) composed of colanic acid (CA). Studies were performed to evaluate the association of production of CA with survival of EHEC under adverse environmental conditions. A CA-deficient mutant, M4020, was obtained from a CA-proficient parental strain, E. coli O157:H7 W6...

Four synthetic oligonucleotide probes representing different regions of the Shiga-like toxin I (SLT-I) structural genes and one oligonucleotide derived from the SLT-II gene of Escherichia coli serotype O157:H7 strain 933 were examined for the identification of E. coli strains that produce cytotoxins for Vero or HeLa cells. E. coli strains that synthesize SLT-I alone or O157:H7 isolates that coe...

The significance of Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 as the major cause of hemorrhagic colitis and the hemolytic-uremic syndrome (HUS) worldwide has been well established (for reviews, see references 21, 31, 41, and 60). The recognition of this pathogen has been facilitated by the availability of classical microbiological diagnostic procedures that are based on the ch...