We have examined the capacity of the major secretory protein (MSP) of Legionella pneumophila to induce humoral, cell-mediated, and protective immunity in a guinea pig model of Legionnaires' disease. MSP was purified to homogeneity by ammonium sulfate precipitation, molecular sieve chromatography, and ion-exchange chromatography. The purified MSP was nonlethal and nontoxic to guinea pigs upon su...

tIapten conjugates of synthetic poly amino adds are useful tools for the investigation of the metabolism of antigen, which is an early step in the immune response. Previous studies have shown that approximately 25 per cent of random-bred guinea pigs and 100 per cent of the highly inbred strain 2 guinea pigs are capable of developing an immune response to lightly coupled hapten-poly-L-lysine (PL...

This study describes two experiments that were conducted in search for a behavioral paradigm to test for tinnitus in guinea pigs. Conditioning paradigms are available to determine the presence of tinnitus in animals and are based on the assumption that tinnitus impairs their ability to detect silent intervals in continuous noise. Guinea pigs have not been subjected to these paradigms yet; there...

HBs vaccines were prepared by three different methods: 1) Heat inactivation (Krugman, et.al 1971)2) Ammonium sulphate precipitation followed by column chromatography (Sephadex G-200) 3) PEG-6000 (polyethylene glycol) precipitation followed by column chromatography using Sephadex G-200. Their efficacy was studied in guinea pigs, and the results compared with commercial Japanese vaccine (Gr...

An immune response (fluorescent antibody to membrane antigen) was detected in guinea pigs inoculated with varicella-zoster virus (VZV) adapted to guinea pig embryonic cells, including the Oka vaccine strain, even when inoculation was by an external route, i.e., nasal or corneal. Live or UV-inactivated virus having the same virus titer before irradiation was administered to guinea pigs by the co...

There are important species-specific differences in K+ current profiles and arrhythmia susceptibility, but interspecies comparisons of K+ channel subunit expression are lacking. We quantified voltage-gated K+ channel (Kv) subunit mRNA and protein in rabbits, guinea pigs, and humans. Kv1.4, Kv4.2, and Kv4.3 mRNA was present in rabbits but undetectable in guinea pigs. MinK mRNA concentration in g...

The presence of endogenous oncornavirus and herpesvirus in guinea pigs has been established. The oncornavirus apparently is present in all guinea pigs but is expressed only under certain conditions. Expression of the latent herpesvirus is generally age and strain dependent as is the development of spontaneous guinea pig leukemia. Following special laboratory manipulation, expression of both vir...

1. Infection with tubercle bacilli of bovine type produced no alteration in the serum cholesterol content in guinea pigs. 2. Certain controlled variations in diet produced no changes in the serum cholesterol content in guinea pigs. 3. Inbred families of guinea pigs known to manifest differing resistances to tuberculosis gave differing serum cholesterol values, but no direct relationship between...

Chemically modified hemoglobin (Hb) solutions are promising oxygen therapeutics; however, these agents are prone to intravascular oxidation. Using a 50% exchange transfusion (ET) model with bovine polymerized hemoglobin (PolyHbBv), we examined heme oxidation, oxygenation markers, and toxicokinetics in rats, an ascorbic acid (AA)-producing species, and in guinea pigs, a non-AA-producing species....

Effects of R[+]-8-([1-[3,4-dimethoxyphenyl]-2-hydroxyethyl]amino) -3,7-dihydro-7-[2-methoxyethyl]-1,3-dimethyl-1H-purine-2,6-dione (MKS-492), a reported type III isozyme inhibitor of cyclic nucleotide phosphodiesterase, on antigen- or platelet activating factor (PAF)-induced bronchoconstriction and allergic reactions in guinea pigs and rats were investigated. 1) MKS-492 inhibited antigen-induce...