The physicochemical properties of the antileukemic Lasparaginase from Erwinia carotovora have been studied and compared with those of the corresponding clinically effective enzyme from Escherichia coli B. Although both enzymes are tetramers with similar molecular weight, the amino acid compositions are distinctly different and the Erwinia enzyme is more basic as judged by polyacrylamide electro...

Pig gastric mucins were isolated from the surface epithelium of the cardiac region, corpus and antrum using density-gradient centrifugation after extraction in 6 M guanidinium chloride. In CsCl/0.5 M guanidinium chloride, mucins solubilized from the cardiac region appeared as a broad unimodal band at 1.52 g/ml whereas those from the corpus and antrum occurred as high- and low-density population...

BACKGROUND Antibodies are prototypes of multimeric proteins and consist of structurally similar domains. The two variable domains of an antibody (VH and VL) interact through a large hydrophobic interface and can be expressed as covalently linked single-chain Fv (scFv) fragments. The in vitro folding of scFv fragments after long-term denaturation in guanidinium chloride is known to be slow. In o...

Choline-binding modules are present in some virulence factors and many other proteins of Streptococcus pneumoniae (Pneumococcus). The most extensively studied choline-binding module is C-LytA, the C-terminal moiety of the pneumococcal cell-wall amidase LytA. The three-dimensional structure of C-LytA is built up from six loop-hairpin structures forming a left-handed beta-solenoid with four choli...

Reduction of the protein-S-S-R bond in the zymogen of streptococcal proteinase leads to an enzymatically active molecule without any proteolysis being required. This conclusion is based upon experiments in which the zymogen has been reduced in very dilute solution where the rate of bimolecular autodigestion is at a minimum. Under these conditions, even in the presence of 3.5 M guanidinium chlor...

A procedure for the isolation of bovine Factor V has been developed. The Factor V is isolated from bovine plasma by a series of steps including barium citrate adsorption, polyethylene glycol precipitation, QAE-cellulose adsorption, hydrophobic chromatography on octyl Sepharose, ammonium sulfate fractionation, preparative electrophoresis on acrylamide gels, and finally, phenyl Sepharose chromato...

A simple and robust method is presented to immobilize a heparin-analog polyelectrolyte on inert hydrophobic surfaces. It demonstrated that an amphiphilic block copolymer consisting of linear polyglycerol sulfate (lPGS) benzophenone modified anchor can be bound polystyrene surfaces in facile dip-coating procedure. The chaotropic salt guanidinium chloride used overcome the aggregation polymer as ...