Unlike the sigma(70)-controlled P2 promoter for the osmotically regulated proU operon of Escherichia coli and Salmonella enterica serovar Typhimurium, the sigma(s)-controlled P1 promoter situated further upstream appears not to contribute to expression of the proU structural genes under ordinary growth conditions. For S. enterica proU P1, there is evidence that promoter crypticity is the result...

BACKGROUND/AIMS Expression of the human growth hormone (GH) gene (GH1) is regulated by a locus control region (LCR) and the highly polymorphic GH1 promoter. We analyzed GH1 LCR/promoter single nucleotide polymorphisms (SNPs) in patients with isolated growth hormone deficiency (IGHD) in relation to clinical data. METHODS We directly sequenced the GH1 LCR/promoter of 62 Dutch IGHD patients with...

We have previously shown that the activity of the Escherichia coli rRNA promoter rrnB P1 in vitro depends on the concentration of the initiating nucleotide, ATP, and can respond to changes in ATP pools in vivo. We have proposed that this nucleoside triphosphate (NTP) sensing might contribute to regulation of rRNA transcription. To test this model, we have measured the ATP requirements for trans...

BACKGROUND Baker's yeast Saccharomyces cerevisiae is a proven host for the commercial production of recombinant biopharmaceutical proteins. For the manufacture of heterologous proteins with activities deleterious to the host it can be desirable to minimise production during the growth phase and induce production late in the exponential phase. Protein expression by regulated promoter systems off...

Greening of cotyledons during de-etiolation is critical for harvesting light energy and sustaining plant growth. PIF3 HY5 antagonistically regulate protochlorophyllide synthesis in the dark. However, mechanism by which PIF3/HY5 module regulates genes involved not clear. Using genetic, molecular biochemical techniques we identified that B-BOX protein BBX11 acts directly downstream to transcripti...

Light provides energy for photosynthesis and is also an important environmental signal that regulates plant growth development. Ribose-5-phosphate isomerase plays a crucial role in photosynthesis. However, ribose-5-phosphate has yet to be studied soybean To understand the biological function of GmRPI2, this study, GmRPI2 was cloned, overexpression vectors gene editing were successfully construc...

The main objective of this study was to determine the effects of plant growth promoter bacteria on biomass and yield of basil. The experiment was carried out as randomized complete blocks design with eight treatments and three replications at research field of Agriculture Company of Ran in Firouzkuh of iran in 2012 . The treatments were [1] Azotobacter chroococcum (A), [2] Azospirillum lipoferu...

There are two different co-expression systems including bicistronic; dual-vector or two-promoter to express two different genes simultaneously and also to study protein-protein interactions. Bicistronic system has disadvantages e.g. compared with two-promoter system. In this paper, a simple method based on spliced overlap extension by polymerase chain reaction (SOE-PCR) technique was demonstrat...