نتایج جستجو برای: granzyme h

تعداد نتایج: 533304  

2014
Himanshu Kaushal Rachel Bras-Gonçalves Narender Singh Negi Jean-Loup Lemesre Gérard Papierok Poonam Salotra

BACKGROUND Majority of individuals with history of visceral leishmaniasis (VL) exhibit strong immunity to re-infection, however, the mechanism of resistance is poorly understood. It is unclear whether CD8(+) T cells contribute to protection against Leishmania donovani infection through cytotoxic activity. The present study aims to evaluate immunological mechanism associated with resistance to t...

2014
Loredana Cifaldi Giusi Prencipe Ivan Caiello Claudia Bracaglia Raffaele Strippoli Fabrizio De Benedetti

OBJECTIVE Systemic juvenile idiopathic arthritis (JIA) is associated with high levels of interleukin-6 (IL-6) in the serum and synovial fluid, and impairment of natural killer (NK) cell function is often observed. This study was undertaken to evaluate a possible link between these 2 biologic findings and whether they may be associated with the development of macrophage activation syndrome, a co...

Journal: :The Journal of biological chemistry 2004
Sandra Waugh Ruggles Robert J Fletterick Charles S Craik

Granzymes are trypsin-like serine proteases mediating apoptotic cell death that are composed of two genetically distinct subfamilies: granzyme A-like proteases resemble trypsin in their active site architecture, while granzyme B-like proteases are quite distinct. Granzyme B prefers substrates containing P4 to P1 amino acids Ile/Val, Glu/Met/Gln, Pro/Xaa, and aspartic acid N-terminal to the prot...

2011
Luis J. Espinoza Akiyoshi Takami Katsuya Nakata Kayoko Yamada Makoto Onizuka Takakazu Kawase Hiroshi Sao Hideki Akiyama Koichi Miyamura Shinichiro Okamoto Masami Inoue Takahiro Fukuda Yasuo Morishima Yoshihisa Kodera Shinji Nakao

Serine protease granzyme B plays important roles in infections, autoimmunity, transplant rejection, and antitumor immunity. A triple-mutated granzyme B variant that encodes three amino substitutions (Q48R, P88A, and Y245H) has been reported to have altered biological functions. In the polymorphism rs8192917 (2364A>G), the A and G alleles represent wild type QPY and RAH mutant variants, respecti...

Journal: :Respiratory Research 2009
Richard A Urbanowicz Jonathan R Lamb Ian Todd Jonathan M Corne Lucy C Fairclough

BACKGROUND There is mounting evidence that perforin and granzymes are important mediators in the lung destruction seen in COPD. We investigated the characteristics of the three main perforin and granzyme containing peripheral cells, namely CD8+ T lymphocytes, natural killer (NK; CD56+CD3-) cells and NKT-like (CD56+CD3+) cells. METHODS Peripheral blood mononuclear cells (PBMCs) were isolated a...

Journal: :Clinical and experimental immunology 2005
M Huang H Ida M Kamachi N Iwanaga Y Izumi F Tanaka K Aratake K Arima M Tamai A Hida H Nakamura T Origuchi A Kawakami N Ogawa S Sugai P J Utz K Eguchi

The objective of this study was to detect autoantibodies against granzyme B cleavage products in sera from patients with primary Sjögren's syndrome (SS). Cell lysates derived from human salivary gland (HSG) cell lines were incubated with granzyme B. The susceptibility to the generation of cleavage fragments of SS autoantigens was assayed by immunoblotting using sera from 57 primary SS patients,...

Journal: :Molecular cancer therapeutics 2008
Bettina Stahnke Theo Thepen Michael Stöcker Reinhard Rosinke Edgar Jost Rainer Fischer Mehmet K Tur Stefan Barth

Acute myeloid leukemia (AML) cells of subtypes M4 and M5 show enhanced expression of CD64 (FcgammaRI), the high-affinity receptor for IgG, which is normally expressed at high levels only on activated cells of the myeloid lineage. CD64 is therefore a prime target for the specific delivery of cytotoxic agents. A promising toxin candidate is granzyme B, a human serine protease originating from cyt...

Journal: :The Journal of Cell Biology 2003
Alan W. Dove

When death starts with a caspase hen a cytotoxic lymphocyte delivers granzyme B to a target cell, current models emphasize that the enzyme induces apoptosis through a mitochondria-centered pathway, cleaving the proapoptotic Bcl-2 family member Bid to cause mitochondrial permeabilization. On page 875, Metkar et al. overturn this view, showing that in the dominant pathway granzyme B begins by pro...

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