Abortion is the major reproductive problem in goats faced by goat-keepers. animals including caused mainly infectious agents. The current study was planned to assess presence of Brucella melitensis, Chlamydophila spp., Campylobacter abortus and Coxiella burnetii genital tracts foetal stomach contents. A total 260 biological samples (vaginal/preputial secretions contents) were randomly collected...

background: aspergillus species are associated with allergic bronchopulmonary disease, mycotic keratitis, otomycosis, na­sal sinusitis and invasive infection. in this study, we developed a pcr-single strand confomational polymorphism method to identify the most common aspergillus species and we showed some advantages of this method comparing a pcr-restric­tion fragment length polymorphism with ...

We investigated the structure of bacterial communities present in livestock manure-based composting processes and evaluated the bacterial succession during the composting processes. Compost samples were derived separately from swine manure, dairy manure and sewage sludge. The structure of the bacterial community was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (...

Recirculation technology has been emerging in the marine aquaculture industry. The microbiome developed recirculation systems (RASs) is an important factor for optimal operation of these and fish welfare. In this study, microbial community dynamics water column biofilms a RAS with Mediterranean species gilthead sea bream bass were investigated, while physicochemical conditions also monitored. M...

We describe the development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on PCR and denaturing gradient gel electrophoresis (DGGE). Bifidobacterium genus-specific primers were used to amplify an approximately 520-bp fragment from the 16S ribosomal DNA (rDNA), and the fragments were separated in a sequence-specific manner in DGGE. PCR produ...

This study aimed to develop a 16S rRNA PCR-denaturing gradient gel electrophoresis (DGGE) to identify the species level of Lactobacillus casei group and to investigate their characteristics of acid production and inhibitory effect. PCR-DGGE has been developed based on the 16S rRNA gene, and a set of HDA-1-GC and HDA-2, designed at V2-V3 region, and another set of CARP-1-GC and CARP-2, designed ...