BACKGROUND Minimally invasive glucose biosensors with increased functional longevity form one of the most promising techniques for continuous glucose monitoring. In the present study, we developed a novel nanoengineered microsphere formulation comprising alginate microsphere glucose sensors and anti-inflammatory-drug-loaded alginate microspheres. METHODS The formulation was prepared and chara...

Bacillus coagulans 2-6 is an excellent producer of optically pure l-lactic acid. However, little is known about the mechanism of synthesis of the highly optically pure l-lactic acid produced by this strain. Three enzymes responsible for lactic acid production-NAD-dependent l-lactate dehydrogenase (l-nLDH; encoded by ldhL), NAD-dependent d-lactate dehydrogenase (d-nLDH; encoded by ldhD), and gly...

Control of glucose concentration has tremendous significance in medical diagnosis, pharmaceuticals, food, and fermentation industries. Herein, we report on the fabrication a facile, low-cost, sensitive enzyme-based amperometric sensor using electrochemically deposited polyaniline (PANI) film graphite electrode. PANI was from an aqueous solution 0.2 M aniline 1.0 hydrocholoric acid (HCl) by cycl...

The presence of pathogenic bacteria in foods has always been a great threat to the wellbeing of people and the revenue of food manufacturers. Therefore, the demand for advanced detection methods that can sensitively and rapidly detect these pathogens has been of great importance. This study reports an electrochemical biosensor for rapid detection of E. coli O157:H7 with the integration of bifun...

The investigation of enzyme reaction kinetics in nanoconfined spaces mimicking the conditions in living systems is of great significance. Here, a nanofluidics chip integrated with an electrochemical detector has been designed for studying "free state" enzyme reaction kinetics in nanoconfinement. The nanofluidics chip is fabricated using the UV-ablation technique developed in our group. The enzy...

In an ongoing programme to develop characterization strategies relevant to biosensors for in-vivo monitoring, glucose biosensors were fabricated by immobilizing the enzyme glucose oxidase (GOx) on 125 μm diameter Pt cylinder wire electrodes (Pt(C)), using three different methods: before, after or during the amperometric electrosynthesis of poly(ortho-phenylenediamine), PoPD, which also served a...

Poly-5,2':5',2''-terthiophen-3'-carboxylic acid (polyTTCA) and poly-Fe(III)-[N,N'-bis[4-(5,2':5',2''-terthien-3'-yl)salicyliden]-1,2-ethanediamine] (polyFeTSED) were synthesized and electrochemically polymerized on an Au surface for use as mediators and catalysts for a biofuel cell. The atomic force microscopy (AFM) images of (a) the TTCA homopolymer (polyTTCA) and (b) the TTCA-FeTSED copolymer...

P36. Quantifying the Development of Breast Cancer Surgery-Associated Lymphedema in High-risk Patients Undergoing Axillary Lymph Node Surgery in Consecutive Patients The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters. Citation Chattha, Anmol, Parisa Kamali, Charlotte L. Van Veldhuisen, Renata Flecha-Hirsch, Alexandra Buckno...

A polyvinylidene fluoride (PVDF) film-based capacitive biosensor was developed for glucose sensing. This device consists of a PVDF film sandwiched between two electrodes. A capacitive biosensor measures the dielectric properties of the dielectric layers at the interface between the electrolyte and the electrode. A glucose oxidase (GOx) enzyme was immobilized onto the electrode to oxidize glucos...

Well-crystallized zinc oxide nanonails have been synthesized in a large quantity via a thermal evaporation method using the metallic zinc powder without the use of any metal catalyst or additives. The detailed structural and optical characterizations confirmed that the as-synthesized nanonails are single-crystalline with the wurtzite hexagonal phase, grown along the [0001] direction and possess...