Goldfish reproduction is coordinated by pheromones that are released by ovulating females and detected by males. Two highly potent pheromones, a dihydroxyprogesterone and a prostaglandin, previously have been identified, and their effects on goldfish behavior have been studied in depth. We have cloned goldfish olfactory epithelium cDNAs belonging to two multigene G-protein coupled receptor fami...

Apoptosis is a programmed cell death that has been attributed to the action of stress hormones, such as cortisol. We investigated the in vitro effect of cortisol in goldfish (Carassius auratus) mature (GSI: 2.75-4.65) or immature (GSI: 1.94-2.4) testes. Testes were taken out and transferred into culture medium (M 199), cut into 20 mm thick slices and supplemented with fetal bovine serum (FBS), ...

The disruption of memory formation in goldfish with inhibitors of protein synthesis suggests a possible connection between proteins and memoryL Although drugs are convenient tools for an indication of such a relationship, the demonstration of proteins directly involved in the memory process would provide more direct support for this concept. We have previously studied protein metabolism of the ...

The existence of food anticipatory activity (FAA) in animals subjected to daily feeding schedules seems to be mediated by a feeding-entrainable oscillator (FEO). Such an FEO may help in anticipating meal time and so optimizing food acquisition and nutrient utilization. In this study we investigated the existence of FAA and whether digestive enzymes, plasma cortisol, hypothalamic NPY and gastroi...

The present work analyzes the involvement of telencephalon of goldfish in spatial strategies, using a procedure analogue to the hole-board task. With this aim, goldfish with sham operation or telencephalon ablation were trained to find a baited feeder within a twenty-five feeder matrix, which maintained stable spatial relationships relative to five peripheral landmarks. After training, differen...

We investigated the differences in the expression of the neurohormones kisspeptin (Kiss) and gonadotropin-inhibitory hormone (GnIH) and cytochrome P450 aromatase (P450arom), gonadotropin hormones (GTHs), and sex steroids in the goldfish Carassius auratus exposed to light-emitting diodes (LEDs). The expression levels of Kiss1, Kiss2, G-protein-coupled receptor 54 (GPR54), GTHs, GnIH, and P450aro...

Twin-tail goldfish possess a bifurcated caudal axial skeleton. The scarcity of this trait in nature suggests that a rare mutation, which drastically altered the mechanisms underlying axial skeleton formation, may have occurred during goldfish domestication. However, little is known about the molecular development of twin-tail goldfish. Here we show that the bifurcated caudal skeleton arises fro...

BACKGROUND Large volumes of lymph can be collected from the eye-sacs of bubble-eye goldfish. We attempted to induce vitellogenin (Vtg) in the eye-sac lymph of bubble-eye goldfish and develop a method for visualizing Vtg incorporation by zebrafish oocytes using FITC-labeling. METHODS Estrogen efficiently induced Vtg in the eye-sac lymph of goldfish. After FITC-labeled Vtg was prepared, it was ...