A recently developed solid-state NMR method for measurement of depths in membrane systems is applied to gramicidin A, a membrane-bound peptide of known structure, to investigate the potential of this method. (15)N-detected, (1)H spin diffusion experiments demonstrate the resolution of the technique by measuring the 4-5 A depth differences between three (15)N-labeled backbone sites (Trp13, Val7,...

The thioquinolobactin siderophore from Pseudomonas fluorescens ATCC 17400 utilizes a variation of the sulfur transfer chemistry found in thiamine and molydobterin biosynthesis. A JAMM motif protein cleaves the C-terminal amino acid residues following a diglycine moiety on a small sulfur carrier protein, and the modified C terminus is activated and sulfurylated, forming a thiocarboxylate.

In this work, an ultrafast and facile method is developed to synthesize Au(I)-dodecanethiolate nanotubes (Au(I)NTs) with the assistance of glycyl-glycyl-glycine (G-G-G). Transmission electron microscopy (TEM) images reveal that the as-prepared Au(I)NTs can be obtained in a 2-h reaction instead of a previous 24-h reaction and are uniform with a hollow structure and smooth surface by virtue of th...

Golgi preparation. Lactose/glycylglycine eluted a few proteins, especially one of about 70000mol.wt. NaCl eluted several further proteins of group B, of mol.wt. 67000-76000, as well as several proteins of groups C and D. The EDTA extract contained several proteins present in the NaCl extract, but was different from the NaCl extract in that it usually contained three proteins of mol.wts. 38000, ...

Protonated a(2) and a(3) (therefore doubly charged) ions in which both charges lie on the peptide backbone are formed in collision-induced dissociations of [La(III)(peptide)(CH(3)CN)(m)](3+) complexes. Abundant (a(3)+H)(2+) ions are formed from triproline (PPP) and peptides with a proline residue at the N-terminus; these peptides are the most effective in producing ions of the type (a(2)+H)(2+)...

The solubilities of amino acids, diglycine, and triglycine have been measured in water and aqueous ethanol as well as dioxane solutions. Free energies of transfer of amino acid side chains and backbone peptide units from water to ethanol and dioxane solutions have been calculated from these data. The results show the similarity between the ‘effects of ethanol and dioxane on the stability of tho...

aAA ay with no add ed glutam ate repeated at end of experiment. bition at the lower (and suboptimal) substrate concentration used in this method.fect of glutamate on apparent serum-y-glu-tamyltranspeptidase activity. and-y-glutamyltranspeptidase activity. of significant effect of L(+)-glu-tamate on serum-y-glutamyltransferase activity det&rmined in the presence of glycyl-glycine. of-y-glutamyl-...