Aerobic glycolysis has been generally associated with cancer cell proliferation, but fascinating and novel data show that it is also coupled to a series of further cellular functions. In this Mini Review, we will discuss some recent findings to illustrate newly defined roles for this process, in particular in non-malignant cells, supporting the idea that metabolism can be considered as an integ...

Creatine kinase (CK) and glycolysis represent important energy-buffering processes in the cardiac myocyte. Although the role of compartmentalized CK in energy transfer has been investigated intensely, similar duties for intracellular glycolysis have not been demonstrated. By measuring the response time of mitochondrial oxygen consumption to dynamic workload jumps (tmito) in isolated rabbit hear...

Recently, studies of anaerobic glycolysis in our laboratory led to the development of a medium for maintaining phosphat,e bond energy in homogenates of tumor (1). This made possible the study of synthetic reactions requiring such an energy supply. Subsequently it became necessary to have a medium in which homogenates of rat liver would maintain phosphate bond energy under anaerobic conditions. ...

The kinetic parameters of the 10 glycolytic enzymes and glycolytic fluxes were determined for the first time in Ustilago maydis. Enzyme activities in yeast grown in minimal medium and harvested in the stationary stage were twofold higher than those from yeast grown in rich medium. In contrast, in yeast harvested in the exponential stage, the enzyme activities were higher in cells grown in rich ...

Dysfunction of cardiac cells under hypoxia has been identified as an essential event leading to myocytes functional failure. MiRNAs are importantly regulatory small-noncoding RNAs that negatively regulate gene expression through the direct binding of 3'-UTR region of their target mRNAs. Recent studies have demonstrated that miRNAs are aberrantly expressed in the cardiovascular system under path...

PURPOSE OF REVIEW Cancer cells alter their metabolism in order to support their rapid proliferation and expansion across the body. In particular, tumor cells, rather than fueling glucose in the oxidative phosphorylation pathway, generally use glucose for aerobic glycolysis. In this review, we discuss some of the mechanisms thought to be responsible for the acquisition of a glycolytic phenotype ...

Cancer cells use glucose and glutamine as the major sources of energy and precursor intermediates, and enhanced glycolysis and glutamimolysis are the major hallmarks of metabolic reprogramming in cancer. Oncogene activation and tumor suppressor gene inactivation alter multiple intracellular signaling pathways that affect glycolysis and glutaminolysis. N-Myc downstream regulated gene 2 (NDRG2) i...

Flexible tumor metabolism T umor cells can survive the hypoxia in the center of tumor masses by using glycolysis, rather than oxidative phosphorylation, to generate energy. Most tumor cells also stick to glycolysis when normally oxygenated. now report that some tumor cells, despite their preference for glycolysis, nevertheless retain the ability to use oxidative phosphorylation. The Boston team...

Glycolysis is an ubiquitous metabolic pathway. In addition to its catabolic role, glycolysis also serves an anabolic function by providing C3 precursors for the synthesis of fatty acids, cholesterol and amino acids. It is therefore an amphibolic pathway. The difference in metabolic orientation is tissue-specific and depends on the hormonal and nutritional state. Therefore, it is not surprising ...

This study examined the hypothesis that glycolysis is required for functional recovery of the myocardium during reperfusion by facilitating restoration of calcium homeostasis. [Ca2+]i was measured in isolated perfused rabbit hearts by using the Ca2+ indicator 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N',N'-tetraacetic acid (5F-BAPTA) and 19F nuclear magnetic resonance spectroscopy. In nonische...