BACKGROUND Determining the stability of stored samples for assays that were not available at the time of original collection is problematic. To assess sample stability for a relatively new assay of glycated albumin (GA), we first measured GA in fresh samples and in samples stored for 19-23 years. We then compared the regression of the contemporaneous glycohemoglobin (Hb A(1c)) values against th...

Fructosamine measurement, on the other hand, exploits the ability of serum proteins to undergo glycation (glycosylation) in conditions of increased ambient glycemia. Glycated proteins are an accepted measure of intermediate glycemia2,3 as demonstrated by the well established clinical usage of hemoglobin A determinations. Serum proteins are glycated as well, and determinationlc of their values r...

OBJECTIVES Development and validation of a direct enzymatic HbA1c assay that utilizes a single channel on chemistry auto-analyzers without the need to run separate glycated hemoglobin and total hemoglobin assays. DESIGN AND METHODS An enzyme based single channel assay was developed to measure %HbA1c in human whole blood samples. The performance characteristics of the Diazyme Direct Enzymatic ...

BACKGROUND Few studies have examined patients' views, knowledge, and understanding of glycohemoglobin A(1c) (HbA(1c)) testing. We explored such issues in patients with type 1 diabetes and used their statements to estimate analytical quality specifications for HbA(1c) testing. METHODS We recruited 201 patients from a hospital outpatient clinic. A questionnaire was used to collect information o...

We report age-specific glycohemoglobin values for nondiabetic infants, children, and young adults. These values were determined with an ion-exchange "mini-column" in a specially designed and constructed water bath that maintains column temperature at 23 +/- 0.1 degrees C. Two in-house-prepared controls with glycohemoglobin content amounting to 6.36% and 11.87% of total hemoglobin, stored at -20...