BACKGROUND The alterations of the glutathione peroxidase enzyme complex system occur in physiological conditions such as aging and oxidative stress consequent to strenuous exercise. METHODS Authors optimize the spectrophotometric method to measure glutathione peroxidase activity in rat red blood cell membranes. RESULTS The optimization, when applied to age paired rats, both nulligravid and ...

A new method for the determination of glutathione peroxidase activity in erythrocytes was developed. The present method was applied to the measurement of hydrogen peroxide removal rates by glutathione peroxidase in erythrocytes at 70 microM hydrogen peroxide under simulated in vivo conditions. The removal rates by glutathione peroxidase in mouse erythrocytes were twenty-times faster than those ...

The endogenous defenses of the mouse heart against reactive oxygen metabolites were investigated. The activities of three enzymes capable of detoxifying activated oxygen were determined in both the heart and liver; cardiac muscle contains 150 times less catalase and nearly four times less superoxide dismutase than liver. Glutathione peroxidase activities were, however, similar to the two tissue...

BACKGROUND Alpha lipoic acid is a potent antioxidant that plays numerous roles in human health. This study examined the effect of ALA on rat sciatic nerve ischemia reperfusion damage. AIMS Protective effect of alpha lipoic acid (ALA) on sciatic nerve following ischemia-reperfusion in rats was investigated by using light microscopy and biochemical methods. Provided that the protective effect o...

BACKGROUND Inflammatory mediators, including cytokines and reactive oxygen species, are associated with the pathology of chronic liver disease. Hepatocytes are generally considered as targets but not producers of these important mediators. OBJECTIVES To investigate whether cells of hepatocellular lineage are a potential source of various cytokines we estimated the expression and secretion of ...

selenium is an essential element for humans, therefore, adding it to plants is convenient for biofortification. thus, the aim of this work was to analyze experimentally the ability of sodium selenite to increase the concentration of se and modify the antioxidant activity in tomato plants. we used plants of the toro hybrid variety and applied three treatments: 0, 2 and 5 mg l-1 of selenium as so...

subclinical mastitis is considered as one of the most prevalent diseases in dairy cows, causing drasticloss in the dairy industry. oxidative stress, induced by reactive oxygen species (ros), is believed to be aprimary factor in various cattle diseases including mastitis, but there are few studies regarding the role ofros in subclinical mastitis. this study was undertaken to i) study the changes...

Background: Diethylnitrosamine (DENA) is dietary carcinogen. It known as cancer initiator in various organs. The present study investigated the destructive changes of DENA liver and colon possible therapeutic effects doxorubicin (DOX); pyridazine derivative (MDP) lactobacillus casei (LAB) against induced dysplasia colon.Methods: Lactobacillus were tested for their probiotic properties prepared ...