Serum enzyme activity was sequentially determined in 10 consecutive patients with diabetic ketoacidosis, of whom all had increased -glucuronidase activity, eight had increased amylase activity, and four had increased acid phosphatase activity. Activity of amylase and that of the two lysosomal enzymes were poorly correlated, irrespective of whether peak activities or activities of all samples we...

BACKGROUND The objective of the study was to evaluate the additional value of beta-glucuronidase (BGD), a lysosomal enzyme in the analysis of transudative and exsudative pleural effusions, especially between malignant and non-malignant effusions. DESIGN AND METHODS Pleural fluid samples obtained from four respective diagnostic groups: transudates parapneumonic effusions, malignant effusions o...

Small intestinal mucosal injury is a frequent adverse effect caused by nonsteroidal anti-inflammatory drugs (NSAIDs). The underlying mechanisms are not completely understood, but topical (luminal) effects have been implicated. Many carboxylic acid-containing NSAIDs, including diclofenac (DCF), are metabolized to acyl glucuronides (AGs), and/or ether glucuronides after ring hydroxylation, and ex...

I N vertebrate tissues there appears to be a connexion between /3-glucuronidase and the proliferation of cells. This connexion was first noticed by Levvy, Kerr, & Campbell (1948) when they were investigating the effect of toxic compounds on mouse-liver glucuronidase. The early stages of embryonic development are characterized by rapid proliferations of cells and it is, therefore, of some intere...

The relation of lysosomal enzymes to mammary tumor regression was studied in the highly hormone-dependent MTW9 rat mammary carcinoma. Quantities of the enzymes were determined with the use of a histochemical modification of the single radial immunodiffusion method. Major findings were as follows, (a) Acid phosphatase, arylsulfatase, ßgalactosidase, and 0-glucuronidase significantly increased i...

As a result of the work of Levvy et al. (1948), it is no longer necessary to postulate a synthetic role for fl-glucuronidase in the body (Fishman, 1940) in order to explain the changes in the activity of the enzyme which can be produced in various organs. The view that ,B-glucuronidase is not involved in glucuronide synthesis (Levvy, 1948) receives support from the failure of saccharic acid to ...

Polyclonal antibodies to Escherichia coli beta-galactosidase, beta-glucuronidase, and glutamate decarboxylase were used in coagglutination tests for identification of these three enzymes in cell lysates. Enzyme capture assays were also developed for the detection of E. coli beta-galactosidase and beta-glucuronidase. The enzymes were released by using a gentle lysis procedure that did not interf...

An experimental model of haemorrhagic hypotension was standardized using rabbits to investigate the shock lung syndrome over a period of 120 minutes. Acute hypovolaemia was induced by withdrawal of blood under anaesthesia to a mean arterial pressure of 30 +/- 5 mmHg within 10 minutes. The mean leucocyte counts and the release of lysosomal enzymes (acid phosphatase and beta-glucuronidase) in the...

BALB/3T3 fibroblasts (3T3) were observed to secrete latent, pepsin-activatable forms of cathepsin B and cathepsin L as well as an active form of beta-glucuronidase when cultured in the absence of serum. The secretion of these proteins was stimulated by the cation ionophore monensin: cathepsin B, 4.3-fold; cathepsin L, 7.2-fold; and beta-glucuronidase, 3.1-fold. These increases were accompanied ...