BACKGROUND Galactokinase catalyses the first committed step in galactose metabolism, the conversion of galactose to galactose-1-phosphate. Galactokinase deficiency is an extremely rare form of galactosaemia, and the most frequent complication reported is cataracts. Congenital hyperinsulinism (CHI) is a cause of severe hypoglycaemia in the newborn period. Galactosaemia has not previously been re...

3. Maltose and fructose utilization was low in young calves and increased slightly with age. 4. Sucrose and starch were not utilized. 5. Studies with three older pre-ruminant calves (aged 53, 88 and 106 days) in which the carbohydrates were infused into the proximal duodenum showed that glucose, galactose, lactose and xylose all caused marked increases in the level of blood reducing sugar, wher...

The aim. aim of the study was to content polysaccharide fractions depending on dissolution, qualitative composition and quantitative sugars in corn silk, leaves roots.
 Materials methods. To different polysaccharides - water-soluble (WSPS), pectin substances (PS), hemicelluloses A (HC A) B (HM B) used a gravimetric method based extraction with suitable solvent followed by sedimentation wei...

The first committed step in the biosynthesis of L-ascorbate from D-glucose in plants requires conversion of GDP-L-galactose to L-galactose 1-phosphate by a previously unidentified enzyme. Here we show that the protein encoded by VTC2, a gene mutated in vitamin C-deficient Arabidopsis thaliana strains, is a member of the GalT/Apa1 branch of the histidine triad protein superfamily that catalyzes ...

A property of sugar transport into the human erythrocyte is that a sugar with a high affinity for the hypothetical "carrier" will enter the cell at low concentration more rapidly than a sugar with lower affinity for carrier. At high concentration the sequence will be reversed. This behavior is exemplified by glucose, which enters erythrocytes faster than galactose at 0.015 m and slower than gal...

Bacillus subtilis is unable to grow by consuming galactose because it is unable to transport it into the cell. The transcription of galE is not influenced by galactose but is repressed by glucose. Galactose is toxic for galE-negative bacteria because it results in elevated levels of metabolic intermediates. These negative effects are reduced in galK and galT mutants. Glucose is also toxic for g...

We propose determination of the ratio of the rate of galactose metabolism to glucose metabolism by erythrocytes as a screening test for abnormalities in glucose and galactose metabolism. Packed erythrocytes (20 mul) are incubated for 1 h at 37 degrees C in 0.42 ml of a solution comprising phosphate buffer (pH 7.4), 0.4 mg of glucose, 60 mg of methylene blue, and 50 nCi of either [1-14C]glucose ...

The effects of lactose, galactose, glucose, and glucose combined with galactose on the induction of β–galactosidase by IPTG in Escherichia coli strain VC10, growing in a glycerol media, were compared against a control of glycerol plus IPTG. After inducing β– galactosidase production with the IPTG, the cultures were altered with the addition of one of the above carbohydrates or combinations. Thi...

BACKGROUND Production of valuable metabolites by Yarrowia lipolytica using renewable raw materials is of major interest for sustainable food and energy. Galactose is a monosaccharide found in galactomannans, hemicelluloses, gums, and pectins. RESULTS Yarrowia lipolytica was found to express all the Leloir pathway genes for galactose utilization, which encode fully functional proteins. Gene or...