Hyperimmunization of two groups of rabbits with killed Escherichia coli and Bacillus subtilis has resulted in the formation of a serum factor (RFLS) which resembled the human rheumatoid factor. It was a heat-stable protein that migrated electrophoretically in the gamma-beta globulin range and sedimented rapidly with ultracentrifugation. The serologic properties of the RFLS were destroyed by mer...

This study investigated the subcellular location of mung bean (Vigna radiata) 8S globulin in transient expression systems as well as in tobacco (Nicotiana tabacum) BY-2 cells and different tissues from a transgenic Arabidopsis (Arabidopsis thaliana) line stably expressing this storage globulin. When transiently expressed in protoplasts from both BY-2 cells and Arabidopsis suspension cultured ce...

In a previous communication (1) we reported that the sera of intact, -normal rats of the Long-Evans strain, when examined electrophoretically in saline-sodium phosphate buffer at pH 7.4, contained little or none of the protein component usually designated as a-globulin. In contrast, sera taken from rats 21 days after hypophysectomy uniformly showed the presence of this component. Earlier experi...

Serum protein electrophoresis (SPE) demonstrates a monoclonal protein as an M spike in the majority of patients with monoclonal gammopathies. However, in patients with an apparently normal SPE pattern or hypogammaglobulinemia, immunofixation electrophoresis (IFE) can reveal an M protein. We undertook this study to determine the yield of reflex IFE testing in patients with hypogammaglobulinemia ...

Cells from leukemia L1210 and a subline resistant to methylglyoxal-bis(guanylhydrazone) elicited a demonstrable humoral antibody response in DBA/2Ha-DD mice. Using paired-label 7-globulin mixtures containing '3 ' I-labeled normal and '2 5I-labeled immune 7-globulin, we showed that the specific uptake of antiresistant L1210 cell 7-globulin by resistant L1210 cells was consistently higher than th...

Diffuse glomerulonephritis was induced in unilaterally nephrectomized albino rabbits by giving them two massive intravenous injections of purified bovine serum gamma globulin. Morphologically, these renal lesions were similar to those of human acute and subacute glomenilonephritis. Globulin nephritis in rabbits also closely resembles experimental nephritis induced by injection of other foreign ...

The effect of woundingand formation of regenerating wound tissueon the level of the serum proteins was studied in rats on a protein-free diet. It was found that the level of the globulin component having the third greatest electrophoretic mobility, probablypart of the a,-globulin fraction, roseabout 60% within 3 days after wounding and did not begin to return to normal until about 10 days later...

An automated continuous-flow procedure has been developed for simultaneously measuring total serum protein and globulin. The method for total protein is a minor modification of an existing automated method in which the biuret reagent is used. Total globulin is measured by reaction with glyoxylic acid, and standardized with N-acetyltryptophan. An empirical factor relating concentration of N-acet...

Purification of the activity of the fourth component of human complement resulted in the isolation of a highly homogeneous serum protein. Since this protein has not been recorded previously it was called beta(1E)-globulin on the basis of its immunoelectrophoretic behavior. C'4 activity and beta(1E)-globulin were found to have highly similar, if not identical physicochemical characteristics. Mor...