The Sombrero galaxy, NGC 4594, contains the most numerous globular cluster system of any nearby spiral. It is an ideal candidate in which to study the globular clusters and contrast them with those in Local Group spirals. Here we present B and I imaging from the CTIO Schmidt telescope which gives a field-of-view of 31 ′ × 31 ′ . Using DAOPHOT we have detected over 400 globular clusters and deri...

At the distance of NGC 5128 (3.6±0.2 Mpc) it is possible to resolve globular clusters with high resolution imaging from the ground, thus allowing the globular cluster candidate selection primarily through their morphological properties. I report the discovery of 71 globular clusters in NGC 5128 on VLT UT1+FORS1 images, including the faintest members (MV ∼ −5) known to date in this galaxy as wel...

LytB and GcpE, because they are codistributed with other pathway enzymes, have been predicted to catalyze unknown steps in the nonmevalonate pathway for isoprenoid biosynthesis. We constructed a conditional Escherichia coli lytB mutant and found that LytB is essential for survival and that depletion of LytB results in cell lysis, which is consistent with a role for this protein in isoprenoid bi...

There are several different families of repeat proteins. In each, a distinct structural motif is repeated in tandem to generate an elongated structure. The nonglobular, extended structures that result are particularly well suited to present a large surface area and to function as interaction domains. Many repeat proteins have been demonstrated experimentally to fold and function as independent ...

Spatial arrangement of carbon in protein structure is analyzed here. Particularly, the carbon fractions around individual atoms are compared. It is hoped that it follows the principle of 31.45% carbon around individual atoms. The results reveal that globular protein's atoms follow this principle. A comparative study on monomer versus dimer reveal that carbon is better distributed in dimeric for...

BACKGROUND Solutions containing high macromolecule concentrations are predicted to affect a number of protein properties compared to those properties in dilute solution. In cells, these macromolecular crowders have a large range of sizes and can occupy 30% or more of the available volume. We chose to study the stability and ps-ns internal dynamics of a globular protein whose radius is ~2 nm whe...

Characterization of disordered regions in globular proteins constitutes a significant challenge. Here, we report an approach based on C-detected nuclear magnetic resonance experiments for the identification and assignment of disordered regions in large proteins. Using this method, we demonstrate that disordered fragments can be accurately identified in two homologs of menin, a globular protein ...