In digital pathology, various biomarkers (e.g., KI67, HER2, CD3/CD8) are routinely analyzed by pathologists through immuno-histo-chemistry-stained slides. Identifying these on patient biopsies allows for a more informed design of their treatment regimen. The diversity and specificity types images make the availability annotated databases sparse. Consequently, robust efficient learning-based dia...

Introduction: Examining the tooth is considered as foremost segment in research field of oral biology and pathology. For studying various structures like enamel, dentin cementum, ground section teeth used, while decalcified sections are used for soft tissues.
 Objective: The present study was aimed at exploring normal hard tissue parts where partially were stained them with routine hematox...

Background: Hirschsprung disease is a complex genetic disorder of the enteric nervous system (ENS), often called congenital aganglionic megacolon and characterized by the absence of enteric neurons along a variable length of the intestine. The definitive diagnosis of Hirschsprung disease relies on histologic and/or histochemical staining of sections fr...

or in Van Giesen picrofuchsin. As an instance typical of the ease of use and reliability of the stain, a series of slides was stained for different lengths of time in a trial lot of this solution which contained only 10 grams each of ferrous and ferric alum, instead of the 20 as above described. The slides were then counterstained with Van Giesen picrofuchsin (5 minutes) in the usual manner. Th...

INTRODUCTION Standardization and validation of the color displayed by digital slides is an important aspect of digital pathology implementation. While the most common reason for color variation is the variance in the protocols and practices in the histology lab, the color displayed can also be affected by variation in capture parameters (for example, illumination and filters), image processing ...

BACKGROUND We previously observed decreased p53 immunostaining over time in paraffin-embedded sections of ductal carcinoma in situ of the breast of women; these sections had been stored on slides at room temperature. This observation suggests that slide storage adversely affects p53-immuno-staining intensity and could result in spurious negative staining for p53 in patient samples. PURPOSE Th...

OBJECTIVES To determine the reliability of the diagnosis of non-gonococcal urethritis (NGU), and the variation between and within microscopists, from urethral smears at a large London genitourinary medicine clinic. METHODS A senior microscopist (SM) preselected 60 Gram stained urethral smear slides, 20 negative (<5 polymorphs/hpf), 20 low grade NGU (5-20 p/hpf), and 20 high grade NGU (>20 p/h...

the present study describes the changes in expression of hydroxy- carboxylic acid receptor 2 (hca2 receptor) in femoral epiphysis and metaphysis of rats with glucocorticoid-induced osteoporosis (gio). 16 growing male sprauge dawley rats were randomly divided into two equal groups consisting of normal control and rats that were rendered osteoporotic by receiving 0.1 mg/kg/day dexamethasone throu...