Background: Vitrification of oocytes is a fast-freezing technique, which may affect the quality of the human oocyte, and consequently affects the embryo development, pregnancy and birth. The aim of the current study was to investigate the consequence of in-vitro vitrification on maturation status of immature human oocytes, additionally, expression levels of stress, and apoptosis related genes. ...

In the present study, a total of 595 specimens collected monthly in the northern part of Persian Gulf between November 2006 and October 2007 were examined by routine macroscopic and histological techniques. Monthly changes in the gonadosomatic index showed the spawning season extended within 2 peaks, from April- May and September. The maturity stages are morphologically separated according to t...

introduction: the purpose of this study was to evaluate the effect of different doses of retinoic acid on resumption of meiosis, in vitro maturation of oocytes and resulting embryo development in mice. materials and methods: germinal vesicle of nmri female mouse (6-8 weeks old) oocytes were collected from ovaries and cultured in maturation medium mem-α contained: 100 mliu/ml rfsh + 7.5 iu/ml hc...

Ovarian follicle maturation (OFM), including the resumption of meiosis in oocyte, is under hormonal regulation. Insulin-like growth factors (IGF) have been shown to participate regulation OFM with species-specific actions teleost fishes. In present study, vitro treatment rainbow trout ovarian follicles recombinant human insulin-like factor-1 (rhIGF1) or rhIGF2 did not induce germinal vesicle br...

Objectives: The frequency of aneuploidies in human oocytes is extremely high. It is hypothesized that the cause may be due to abnormal chromatin (histone) acetylation/deacetylation. The aim of our study was to analyzed the acetylation/deacetylation pattern in spare human oocytes. Materials and Methods: Human spare oocytes (311), in other words oocytes that were not mature when collected from fo...

To investigate the protein profiling of buffalo oocytes at the germinal vesicle (GV) stage and metaphase II (MII) stage, an iTRAQ-based strategy was applied. A total of 3,763 proteins were identified, which representing the largest buffalo oocytes proteome dataset to date. Among these proteins identified, 173 proteins were differentially expressed in GV oocytes and competent MII oocytes, and 14...

Roscovitine, a potent inhibitor of M-phase Promoting Factor (MPF) kinase activity, was used to maintain cattle oocytes at the germinal vesicle stage for a 24-hr culture period. A concentration of 25 microM of roscovitine was sufficient to reach the maximum level of meiotic resumption inhibition with 83 +/- 6% of the oocytes remaining at the germinal vesicle stage after the 24 hr of culture. The...