This study investigated the genetic diversity of morchella species using randomly amplified polymorphic DNA markers. Morchella species are an important group of edible mushrooms belonging to the family Helveliaceae with medicinal and economical significance. In this study we have developed an efficient method of genomic DNA isolation which was amplified by eight RAPD primers to test the polymor...

background and objective: formalin-fixed paraffin-embedded tissues are a valuable source of dna for molecular studies. we designed and optimized an efficient procedure for dna extraction from formalin-fixed paraffin embedded tissues. materials and methods: seventy three blocks of cervical paraffin-embedded tissues were investigated. dna was extracted using 45 minutes boiling in alkaline solutio...

allelopathy is one of the valuable tools in weed management that recently is considered in sustainable agriculture and reduces herbicide rate challenges. in order to study the allelopathic effects of rye (secale cereal l.) on six weed spices seeds including milk thistle (silybum marianum l.) gaertn, wimmer ryegrass (lolium rigidum gaudin), lesser canarygrass (phalaris minor retz.), charlock mus...

Rye (Secale cereale L.) serves as an alternative host of Pyrenophora tritici-repentis (PTR) the cause of tan spot on wheat. Rye is cultivated as a forage or cover crop and overlaps with a significant portion of wheat acreage in the U.S. northern Great Plains; however, it is not known whether the rye crop influences the evolution of PTR races. We evaluated a global collection of 211 rye accessio...

mutations in the gjb2 gene are the most common known cause of hereditary congenital hearing loss. rapid genomic dna extraction (rgde) method was used for genomic dna extraction. after amplification of coding region of cx26 gene with specific primers, expected pcr products with 724bp length were subjected to direct sequencing in both directions. we describe here a novel heterozygous -t to -c tra...

The incorporation of a rye (Secale cereale L.) cover crop into the soil prior to planting cotton (Gossypium hirsutum L.) has been shown to restrict damage caused by root-knot nematodes (Meloidogyne incognita (Kofoid and White) Chitwood). A greenhouse study was conducted to determine the duration of the effectiveness of rye decomposition in controlling root-knot nematode damage in relation to th...

CYP3A4 probably has the broadest catalytic activity of any cytochrome P450. It is a crucial task to test new drug candidates in a reliable system for their ability to induce expression of this enzyme. Firstly, a total of 300 bp core distal enhancer of CYP3A4 XREM region (-7972/-7673) were amplified from human genomic DNA. The PCR product was then ligated into a human secretory alkaline phosphat...

Background Genomic imprinting is a heritable and developmentally essential phenomenon by which gene expression occurs in an allele-specific manner1. While the imprinted alleles are primarily silenced by DNA methylation, it remains largely unknown how methylation is targeted to imprinting control region (ICR), also called differentially methylated region (DMR), and maintained. Here we show that ...

Celiac disease is the inability of the small intestine to tolerate some cereal prolamin with sequence specific oligopeptide. Wheat gliadin, rye secalins and barley hordenine are the main proteins involved in the disease mechanisms and may provoke an inflammatory process to damage the small intestine. There are different methods to overcome the problems for such patients one of them is using glu...

BACKGROUND Several studies report that dietary fibre from different sources promotes the feeling of satiety and suppresses hunger. However, results for cereal fibre from rye are essentially lacking. The aim of the present study was to investigate subjective appetite during 8 h after intake of iso-caloric rye bread breakfasts varying in rye dietary fibre composition and content. METHODS The st...