background :polymerase chain reaction (pcr) assay has widely used for the detection of tuberculosis (tb). this study tried to compare in-house pcr with some well-known commercial pcr kits for detection of tb agent. methods : clinical samples obtained from 620 tb suspected patients were analyzed for the diagnosis of mycobacterium tuberculosis complex (mtc) by in-house pcr. all samples were obtai...

background the 30 kda major secretory protein of mycobacterium tuberculosis (antigen 85b) is a primary vaccine candidate. this secreted antigen induces a protective immune response and stimulates the production of ifn-γ in animal models. objectives the aim of this study was cloning and expression of ag 85b of m. tuberculosis in escherichia coli. materials and methods to produce recombinant ag85...

background: tuberculosis is one of the infectious diseases worldwide that has resurgence by the aids epidemic and led to the rise of drug-resistant tuberculosis patients. thus, it seems essential to monitor the drug susceptibility in tuberculosis patients. the new high resolution melting (hrm) method is simple, rapid and inexpensive for detection of the mutations responsible for drug resistance...

conclusions we concluded from the results that the frequency of emb-resistant m. tuberculosis cases in iran is lower than that of many other regions. the pcr-sscp technique can separate resistant isolates from sensitive isolates. the sequencing results of this study showed mutation in codons 309 and 299 of the embb gene. in none of the resistant isolates, mutation was observed in codon 306. fur...

background mycobacterium tuberculosis is a vicious microbe co-existing with the infected host. this pathogen exploited opportunities to spread during periods of urbanization and social upheaval, and got retreated with improved hygiene. objectives this investigation was designed to clone and characterize m. tuberculosis mutt gene, a homologue of a dna repair protein in escherichia coli. the aim ...

Objective: The Rifampicin resistance and susceptibility of Mycobacterium tuberculosis are caused by mutations in the 81-base pair region of the rpoB gene encoding the b-subunit of RNA polymerase. Methods: Isoniazid resistance of M. tuberculosis is related to mutations in inha , oxyR and ahpC genes which 30 to 90 percent of Isoniazid resistance is occurred in 3015 codons of kat...

conclusions pcr was the most sensitive, accurate and rapid method for diagnosis of tb. it was concluded that, for the diagnosis of pulmonary tuberculosis, pcr directly from sputum may be recommended in bangladesh, especially in suspected tuberculosis patients who remain negative by conventional methods. patients and methods a total of 150 clinically suspected pulmonary tb patients (male/female:...

Female genital tuberculosis remains as a major cause of tubal obstruction leading to infertility, especially in developing countries. The global prevalence of genital tuberculosis has increased during the past two decades due to increasing acquired immunodeficiency syndrome. Genital tuberculosis (TB) is commonly asymptomatic and it is diagnosed during infertility investigations. Despite of rece...

Objective: The Rifampicin resistance and susceptibility of Mycobacterium tuberculosis are caused by mutations in the 81-base pair region of the rpoB gene encoding the b-subunit of RNA polymerase. Methods: Isoniazid resistance of M. tuberculosis is related to mutations in inha , oxyR and ahpC genes which 30 to 90 percent of Isoniazid resistance is occurred in 3015 codons of kat...

Mycobacterial species fill a wide variety of evolutionary niches. The most vigorously studied member of the genus is Mycobacterium tuberculosis, the causative agent of human tuberculosis (13). M. tuberculosis was responsible for an estimated 1.6 million deaths worldwide in 2002, making it second to only HIV in mortality attributed to an infectious disease (2). Mycobacterium bovis and Mycobacter...