Conversion of fossil fuels to electrical power is the most popular method of electrical power generation. Due to the depletion of fossil fuels and the increase in air pollution, the necessity of using high efficiency power generation systems is increasing. High temperature fuel cells, such as solid oxide fuel cells (SOFC) and molten carbonate fuel cells (MCFC), have high efficiency. According t...

Epithelial cells line the lumens of organs including the gastrointestinal tract, kidney tubules and respiratory airways, where they regulate the transport of electrolytes and the movement of macromolecules. The current study aimed to investigate the transport of IGF-I across epithelial cell barriers. Epithelial cell lines derived from gut (IEC-6), kidney (MDBK) and lung (Mv1Lu) were shown to po...

Initiation of herpesvirus infection requires attachment of virions to the host cell followed by fusion of virion envelope and cellular cytoplasmic membrane during penetration. In several alphaherpesviruses, glycoprotein C (gC) is the primary attachment protein, interacting with cell-surface heparan sulfate proteoglycans. Secondary binding is mediated by gD, which, normally, is also required for...

As an immunotoxin, diphtheria toxin has been widely used in gene therapy and gene function assays for its roles in protein synthesis inhibition, and the aim of our study is to set up a nonintegrating lentiviral system for specific expression of diphtheria toxin A (DTA) used in cancer gene therapy.Here, we established a lentiviral system that could coordinately express fluorescent protein and DT...

Started in 1993, the Debian project is one of the oldest Free Software projects. Due to its volunteer nature, specialists from all fields contribute to the Debian GNU/ Linux distribution, which includes more than 30000 packages. The Debian packaging policy, its advanced package management system and the conservative release process lead to a stable basis which is ideal for customized environmen...

A low molecular mass protein which we term S100L was isolated from bovine lung. S100L possesses many of the properties of brain S100 such as self association, Ca++-binding (2 sites per subunit) with moderate affinity, and exposure of a hydrophobic site upon Ca++-saturation. Antibodies to brain S100 proteins, however, do not cross react with S100L. Tryptic peptides derived from S100L were sequen...

The packaging signal (psi) of human immunodeficiency virus type 2 (HIV-2) is present in the 5' noncoding region of RNA and contains a 10-nucleotide palindrome (pal; 5'-392-GGAGUGCUCC) located upstream of the dimerization signal stem-loop 1 (SL1). pal has been shown to be functionally important in vitro and in vivo. We previously showed that the 3' side of pal (GCUCC-3') is involved in base-pair...

BACKGROUND Human immunodeficiency virus type 1 (HIV-1)-based gene delivery systems are popular due to their superior efficiency of transduction of primary cells. However, these systems cannot be readily used for delivery of anti-HIV-1 genes that target constituents of the packaging system itself due to inimical effects on vector titer. Here we describe HIV-1-based packaging systems containing t...

Self-heating ignition is a fire hazard in warehouses when stacking large quantities of reactive materials for storage, including lithium-ion batteries. Due to the heavy costs and dangerous risks, thermal behaviour large-scale LIB ensembles usually studied by numerical methods. The state-of-the-art self-heating models on LIBs are either too computationally expensive be applied predictions ensemb...