REPORTS Recombinant DNA: Examples ofPresent-Day Research:J.Abelson. Charon Phages: Safer Derivatives ofBacteriophage Lambda forDNA Cloning: F. R. Blattner et al. . Recombinational Switch for Gene Expression: J. Zieg et al. Gene Cloning and Containment Properties of Plasmid Col El and Its Derivatives: K. A. Armstrong, V. Hershfield, D. R. Helinski. EK2 Derivatives ofBacteriophage Lambda Useful i...

With the sequencing of genomes from many organisms now complete and the development of high-throughput sequencing, life science research has entered the functional post-genome era. Therefore, deciphering the function of genes and how they interact is in greater demand. To study an unknown gene, the basic methods are either overexpression or gene knockout by creating transgenic plants, and gene ...

There are many ways to isolate a DNA fragment containing a specific gene. The methods vary depending on the organism and the genetic and recombinant DNA technology available for working with that particular organism. Moreover, the approach chosen will vary depending on several other factors. These include the phenotype of strains carrying the mutant allele, whether the mutation is dominant or r...

background and objective: the outer membrane protein w ( ompw ) of vibrio cholerae is involved in stimulating the im- mune response via induction of protective immunity. it also plays an important role in bacterial pathogenesis by increasing the adaptability of pathogenic strains. in this study we aimed to clone v. cholerae ompw gene in the strain x-33 of pichia pastoris. materials and methods:...

The future development of medicine will greatly depend on research, knowledge and clinical application of stem cells. In recent years, stem cells especially; skin stem cells have been very interesting and amazing targets for study and scientific application in the following fields : 1- Cell-based therapy; 2- Regenerative medicine and tissue engineering; 3- Gene therapy; 4- Therapeutic cloning; ...

objective: the clavulanic acid regulatory gene (clar) is in the clavulanic acid biosynthetic gene cluster that encodes clar. this protein is a putative regulator of the late steps of clavulanic acid biosynthesis. the aim of this research is the molecular cloning of clar, isolated from the iranian strain of streptomyces clavuligerus (s. clavuligerus). materials and methods: in this experimental ...