Strain PA14, a human clinical isolate of Pseudomonas aeruginosa, is pathogenic in mice and insects (Galleria mellonella). Analysis of 32 different PA14 mutants in these two hosts showed a novel positive correlation in the virulence patterns. Thus, G. mellonella is a good model system for identifying mammalian virulence factors of P. aeruginosa.

Nematodes represent an especially abundant and species-rich phylum, with many free-living and parasitic species. Among the diversity of parasitic species is a guild of specialists known as entomopathogenic nematodes due to their unusual ability to quickly kill their hosts with the aid of pathogenic bacteria. Herein I discuss in detail the hallmarks of entomopathogenic nematodes and how they are...

We report the isolation of a bacterium from Galleria mellonella larva and its identification using genome sequencing and phylogenomic analysis. This bacterium was named Alcaligenes faecalis strain MOR02. Microscopic analyses revealed that the bacteria are located in the esophagus and intestine of the nematodes Steinernema feltiae, S. carpocapsae, and H. bacteriophora. Using G. mellonella larvae...

Defense peptides play a crucial role in insect innate immunity against invading pathogens. From the hemolymph of immune-challenged greater wax moth, Galleria mellonella (Gm) larvae, eight peptides were isolated and characterized. Purified Gm peptides differ considerably in amino acid sequences, isoelectric point values and antimicrobial activity spectrum. Five of them, Gm proline-rich peptide 2...

entomopathogenic fungi produce secondary metabolites which may bioactively help fungus in its virulence toward insect hosts. beauveria bassiana produces several toxic low molecular compounds in vitro as well as in vivo, the most important of them is beauvericin. the beh isolate of the fungus was selected for beauvericin assay. beauvericin was obtained from surface and submerged cultures of the ...

The hemolymph juvenile hormone binding protein (JHBP) from Galleria mellonella contains two disulphide bridges/molecule and no free Cys residues. An alignment of primary structures of other Lepidopteran JHBPs indicates that Cys residues, equivalent to Cys10,17,151,195 in G. mellonella JHBP, maybe involved in -S-S- bridge formation.

Abstract Background Studying the diversity of indigenous entomopathogenic fungi (EPF) is a prerequisite to effective insect pest control. The distribution and occurrence EPF from soil across Nigeria were evaluated. Three subsoil samples three locations in each 11 states used isolate by bait method. fungal rates, 1.0 × 10 2 , 5.0 1 7.5 spores/ml, applied Galleria larvae. Results occurred 41.1% s...

Entomopathogenic nematodes (EPNs) employ a sophisticated chemosensory apparatus to detect potential hosts. Understanding the molecular basis of relevant host-finding behaviours could facilitate improved EPN biocontrol approaches, and could lend insight to similar behaviours in economically important mammalian parasites. FMRFamide-like peptides are enriched and conserved across the Phylum Nemato...