The present study aimed to produce and pathophysiologically evaluate the metallothionein (MT) fusion protein. A recombinant plasmid containing DNA segment coding the pET-glutathione transferase (GST)-small ubiquitin-related modifier (SUMO)-MT fusion protein was inserted into Escherichia coli for expression. The expression level of the fusion protein was very high, reaching to 38.4% of the total...

OBJECTIVES Aging is an unconscious and gradual process that can lead to changes in biological systems. Induction of oxidative stress and apoptosis, hepatotoxicity and neurotoxicity are involved in the aging process. Regarding the antioxidant property of black seed oil, the aim of this study was to evaluate the anti-aging effect of Nigella sativa (N. sativa) oil on d-galactose-induced aging in m...

Glucose-i-phosphate (G-1-P) uridylyl transferase, E.C. 2.7.7.9,' catalyzes the reversible synthesis of uridinediphosphoglucose (UDPG) from G-1-P and uridine triphosphate with the liberation of pyrophosphate. This reaction appears to be ubiquitous in nature.! The importance of this enzyme is evident from its participation in the metabolic pathways of glycogen synthesis,' galactose conversion to ...

LTHOUGH great strides have been made in the past decade in both cytogenetics and biochemical genetics, the location of genes on the 44 autosomes has remained unknown. The experiments of nature in which an additional chromosome-21 is present and where a portion of this chromosome has been deleted have provided, for the first time, a clue to the possible location of some autosomal genes. The stud...

The soluble galactosyltransferase of human plasma catalysed the transfer of galactose from UDP-galactose to high- and low-molecular-weight derivatives of N-acetylglucosamine, forming a beta-1-4 linkage. The enzyme was purified by using (NH4)2SO4 precipitation and affinity chromatography on an alpha-lactalbumin-Sepharose column. The galactosyltransferase was maximally bound to this column in the...

The association of plasmids with virulence characters and O-antigen expression was studied in two virulent and seven avirulent mutant strains of Shigella dysenteriae type 1. Deletion of a 12-Mda segment from a 140-Mda plasmid in two smooth avirulent mutants made the derivatives avirulent in the Sereny test and non-invasive in HeLa cells. The mutants were unable to bind Congo red, and did not ex...

1. Extracts prepared from Epidinium ecaudatum by two disintegration procedures contain oc-galactosidase and isomaltase activities. Nearly all of the oc-galactosidase is extracted by water. 2. The oc-galactosidase hydrolyses galactosylgalactosylglycerol faster than it does melibiose and hydrolyses other compounds containing m-(1l-6)linked galactose more slowly than melibiose. 4-aGalactosylgalact...

background: high content of phospholipids in testes and epididymis is involved in regulation of spermatogenesis. on the other side, it makes testes susceptible to oxidative stress due to lipid peroxidation, which alters the normal mechanism of spermatogenesis. objective: in the present investigation, antioxidant effects of ethanolic extracts of parsley, lettuce and brahmi were tested against d-...

This study was conducted to evaluate the effect of Purslane hydro alcoholic extract on blood urea nitrogen (BUN), creatinine (Cr) and kidney histopathology of kidney aging model induced by D-galactose in female mice. In this experimental study, 72 female Naval Medical Research Institute (NMRI) mice (30-35 g) were randomly divided into six groups including: control, Purslane, D-galactose, D-gala...