We have examined the influence of RNA upon the interaction of Gag-Pol with Gag during human immunodeficiency virus type 1 (HIV-1) assembly. COS7 cells were transfected with protease-negative HIV-1 proviral DNA, and Gag/Gag-Pol complexes were detected by coimmunoprecipitation with anti-integrase. In COS7 cells, Gag/Gag-Pol is found almost entirely in pelletable, membrane-bound complexes. Exposur...

Patients with severe gag reflex (SGR) have difficulty getting the treatment they require in local clinics, and many tend to postpone the start of their treatment. To address this problem, dentists have used behavioral techniques and/or pharmacological techniques for treatment. Among the pharmacological methods available, propofol IV sedation is preferred over general anesthesia because it is a ...

introduction:dystonia is a disorder of movement caused by various etiologies. laryngeal dystonia is caused by the spasm of laryngeal muscles. it is a disorder caused by vocal fold movement in which excessive adduction or abduction of the vocal folds occurs during speech. the pathophysiology of this type of dystonia is not fully known. some researchers have suggested that basal ganglia structure...

A critical aspect of viral replication is the assembly of virus particles, which are subsequently released as progeny virus. While a great deal of attention has been focused on better understanding this phase of the viral life cycle, many aspects of the molecular details remain poorly understood. This is certainly true for retroviruses, including that of the human immunodeficiency virus type 1 ...

Bu çalışmada tavuk sternumundan enzimatik hidrolizasyon yöntemiyle sülfatlanmış glikozaminoglikan (GAG) ekstraksiyonu gerçekleştirilmiş ve optimum işlem koşulu belirlenmiştir. İşlem koşullarının optimizasyonu için Yanıt Yüzey Yöntemi kullanılmıştır. Enzim miktarı, pH değeri, ekstraksiyon sıcaklığı süresi olarak seçilen dört parametrenin GAG verimi üzerindeki etkilerini açıklamak Merkezi Kompozi...

A 26-year-old female presented with epidermolysis bullosa prurogompsa (EBP) since infancy, hair loss, proximal motor weakness, nasal tone speech, swallowing problem, and droopy eyes at age 20. Upon neurological examination, normal cognition, inability to walk unassisted, bilateral ptosis full range of extraocular movements, weak gag reflex, generalized muscle atrophy, decreased deep tendon refl...

The intracellular trafficking and subsequent incorporation of Gag-Pol into human immunodeficiency virus type 1 (HIV-1) remains poorly defined. Gag-Pol is encoded by the same mRNA as Gag and is generated by ribosomal frameshifting. The multimerization of Gag and Gag-Pol is an essential step in the formation of infectious viral particles. In this study, we examined whether the interaction between...

Glycosaminoglycan (GAG) has a pivot role in renal function and homeostasis. Analysis of GAG amount generally serves to determine GAG alteration due to diabetes mellitus. Critical Electrolyte Concentration (CEC) staining can be an efficacy method to study GAG amount changes. Based on an experimental study, 20 male rats were randomly divided equally into two experimental and control groups. Diabe...

A displeasing dental appearance may have a significant emotional impact on an individual's well being. Although malocclusions occur more often in physically and/or mentally handicapped children than in normal children, the most severely handicapped patients are those least likely to receive orthodontic treatment. This investigation studied the modes of behaviour management used in the orthodont...