The severity of cystic fibrosis (CF) pulmonary disease is not directly related to CFTR genotype but depends upon several parameters, including neutrophil-dominated inflammation. Identification of agents modulating inflammation constitutes a relevant goal. Myeloperoxidase (MPO) is involved in both microbicidal and proinflammatory neutrophil activities. The aim of this study was to evaluate wheth...

Abstract Eosinophils and neutrophils are heterogenous cells that play diverse roles in homeostasis inflammation. Tissue eosinophil origins poorly understood, posing challenges the functional interpretation of heterogeneous phenotypes. New evidence from our lab shows unexpected hematopoietic plasticity Ly6G(+)IL-5Rα(+) band-stage with potential to contribute lineage. Using flow cytometry multi-o...

Objective(s): Granulocyte-colony stimulating factor (G-CSF) is used in clinical practice for the treatment of neutropenia and to stimulate generation of hematopoietic stem cells in bone marrow donors. In the present study, the ability of G-CSF in mobilizing exogenous bone marrow stem cells (BMSCs) from peripheral blood into the brain was tested. We for the first time injected a small amount of ...

background & aim: spinal cord injury (sci) is one of the worst kinds of traumatic injuries with remarkable social and economic effects on communities.  methods & materials/patients: in this prospective randomized clinical trial, 122 patients with traumatic spinal cord injury were admitted to poursina hospital within 48 hours of injury to compare granulocyte colony stimulating factor (g-csf) and...

In the present study, we have investigated the involvement of the cyclic adenosine monophosphate (cAMP)-dependent signaling pathway on interleukin-4 (IL-4) gene expression in freshly isolated human T lymphocytes. 2'-0-dibutyryl cAMP (db-cAMP) and prostaglandin E2 (PGE2) were used to directly and indirectly activate the protein kinase A pathway. Northern analysis showed that concanavalin A (Con ...

Mice lacking granulocyte colony-stimulating factor (G-CSF) were generated by targeted disruption of the G-CSF gene in embryonal stem cells. G-CSF-deficient mice (genotype G-CSF-/-) are viable, fertile, and superficially healthy, but have a chronic neutropenia. Peripheral blood neutrophil levels were 20% to 30% of wild-type mice (genotype G-CSF+/+) and mice heterozygous for the null mutation had...

background: recently, the use of granulocyte colony-stimulating factor (g-csf) has been proposed to improve pregnancy outcomes in reproductive medicine. objective: a systematic review of the current use of g-csf in patients who have difficulty conceiving and maintaining pregnancy was performed. materials and methods: two electronic databases (pubmed/ medline and scopus) were searched. study sel...