This study determines the effect of different commercial diluents on quality frozen Simmental semen. The extraction fresh semen from 3 bulls using an artificial vaginal method was performed twice a week. Fresh with motility >70% further processed to one-step dilution method, and used were Andromed®, Optixcell®, Steridyl®. diluted packed into mini-straws, equilibrated, frozen, stored for test...

This study was aimed to evaluate the effects of dietary n-6:n-3 ratio and Vitamin E on the membrane properties and motility characteristics of spermatozoa in boars. Forty Duroc boars were randomly distributed in a 2 × 2 factorial design with two n-6:n-3 ratios (14.4 and 6.6) and two Vitamin E levels (200 and 400 mg kg-1 ). During 16 weeks of treatment, fresh semen was collected at weeks 0, 8, 1...

Research up to 1993 is reviewed. Various methods for processing, freezing and thawing of semen have been elaborated and their effect on survival and fertility of spermatozoa have been examined in vitro or in fertility tests. Diluents initially used for freezing bull semen had their limitations and were modified or replaced by new media. The freezing diluents investigated included citrate-sugar-...

Introduction Although dog was the first species to be used for Artificial Insemination (AI) and become pregnant by Spallanzani in 1780, very little work has been done on preservation of dog semen to exploit the full genetic potential of pedigree males. Frozen semen is now inseminated routinely in cattle, sheep and goats and much research has been carried out with regard to the optimum condition...

bovine leukemia provirus: evidence of presence of part of gag gene inseminal plasma of naturally infected bullsit is of critical importance to understand the modalities of blv presence in semen, especially with regard to artificial insemination (ai). presence of bovine leukemia provirus was demonstrated in fresh and frozen semen samples by researchers. in this study paired blood and semen sampl...

The aim of this study was to evaluate the effect of two different extenders (Skimmed Milk Glucose SMG or Lactose Egg Yolk LEY) on physical characteristics and fertility of fractionated donkey semen cooled at 5°C. For this, four Pêga donkeys were used as semen donors. The sperm rich fraction of the ejaculate was diluted preparing insemination doses containing 400 x 10 6 motile spermatozoa in a v...

Artificial insemination (AI) is the preferred method for reproduction in the majority of the intensive pig production systems Worldwide. To this end, fresh extended ready-to-use semen doses are either purchased from AI-centres or produced by boars kept on-farm. For profitable semen production, it is necessary to obtain a maximum amount of high quality semen from each boar. This paper reviews cu...

In mares, a physiological endometritis ensues following the deposition of semen into the uterus. This response is thought to be due to the antigenic nature of spermatozoa, specifically the activation of complement, and may be modulated by the presence of seminal plasma component of the semen. The removal of seminal plasma during cryopreservation of spermatozoa may account for the longer duratio...

Bovine Leukemia ProVirus: Evidence of Presence of Part of Gag Gene in Seminal Plasma of Naturally Infected Bulls It is of critical importance to understand the modalities of BLV presence in semen, especially with regard to artificial insemination (AI). Presence of bovine leukemia provirus was demonstrated in fresh and frozen semen samples by researchers. In this study paired blood and semen sam...

Background: Neospora caninum (N. caninum) is a cyst-forming coccidian parasite closely related to Toxoplasma gondii which has emerged as an important cause of reproductive failure in cattle worldwide. Routes of Neospora transmission include transplacental infection through tachyzoites, ingestion of tissues harbouring cysts and oral uptake of sporozoitecontaining oocysts. Transplacental transmis...