objective: evaluation of the effects of different doses of antioxidant taurine on oxidative stress and human sperm parameters following cryopreservation. materials and methods: the semen of 20 fertile men were divided to 5 aliquot, one part considered as a fresh after analysis of standard semen parameters (motility, abnormal morphology, viability) and protamine deficiency, dna damage and measu...

BACKGROUND Long-chain polyunsaturated fatty acids (PUFAs) of the omega-3 family are important for sperm membrane integrity, sperm motility and viability. There are evidences to suggest that dietary supplementation with omega-3 fatty acids affects reproduction in men and males of different animal species. Therefore, the aim of current study was to investigate changes in the quality parameters of...

The present study was conducted to detect sperm apoptosis in fresh, capacitated and frozen ram semen and to determine its relationship with other seminal parameters as motility and viability. The cryopreservation process induces the plasma membrane disruptions, attended with increasing of phoshpatidylserine molecules expression on the extracellular surface, wherefore some of the live spermatozo...

Insemination at the uterotubal junction is a practical technique that can be used to decrease the number of spermatozoa used per insemination. Video endoscopic insemination allows one to visualize the uterotubal papilla during the insemination and provide higher pregnancy rates than a flexible AI catheter. Further studies are needed to determine if a consistent technique can be developed for de...

Semen cryopreservation in domestic birds has been studied extensively in the past fifty years. However, efficient methods to freeze semen of chicken of different breeds have emerged only in the last decade of the 20 century. Methods using cryoprotectants Glycerol, Dimethyl Acetamide and Dimethyl sulfoxide, slow or rapid freezing-thawing procedures, pellets or ampoules or straws packaging have b...

Single-cell gel electrophoresis (SCGE), or comet assay, has the ability to detect damage at the single cell level and has not been reported for equine sperm. The ability to detect nuclear damage at the single cell level could aid in the advancement of protocols for optimal semen preservation. The goals of these experiments were to adapt this assay for use with equine sperm and to utilize the as...