Expansive soil is widely distributed in seasonally frozen areas worldwide. Due to the special expansion and shrinkage characteristics of expansive related water content, there are potential engineering disasters subgrade slope engineering. To investigate physical mechanical changes within slope, four freeze-thaw cycles tests were performed on models an environmental chamber with ratios 1:1.5, 1...

The aim of this study was to investigate the effects different freezing and thawing methods on physical, chemical, sensory characteristics red shrimp (Aristaeomorpha foliacea). Shell-on shell-off shrimps were subjected three (blast freezing, still cryogenic freezing) (on air, in refrigerator microwave oven) stored for 30 days at -18ºC. Quality control analyses carried out samples after thawing....

Climate change is likely to modify the freezing–thawing cycles in soils and surface waters of permafrost-affected subarctic regions. However, solution chemical composition during ice formation evolution remaining fluids remain very poorly known. Towards a better understanding dissolved (<0.45 µm) organic carbon, as well major trace element behavior permafrost peatland environments, here we p...

This study was conducted to determine chromatin integrity and DNA damage by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and DNA damage. A significant variation was found in semen parameters after thawing. Highly sig...

C-Phycocyanin is a natural blue dye used in food and pharmaceutical industry. In the present study, a simple and efficient method to extract C-phycocyanin from Spirulina platensis wet biomass is reported. The extractions were carried out using six different methods, including chemical (organic and inorganic acid treatment), physical (freezing and thawing, sonication, homogenization) and enzymat...

Dark-grown y-1 mutant cells of Chlamydomonas reinhardtii accumulate protochlorophyllide (Pchlide) in both 635 nanometers (P635) and 650 nanometers (P650) forms. Plastids in these cells lack the normal thylakoid membrane structure except some remnants of membrane vesicles. Using difference spectrophotometry, P635 is shown to be photoconverted to chlorophyllide at 672 nanometers (C672) and P650 i...

Ectotherms overwintering in temperate ecosystems must survive low temperatures while conserving energy to fuel post-winter reproduction. Freeze-tolerant wood frogs, Rana sylvatica, have an active response to the initiation of ice formation that includes mobilising glucose from glycogen and circulating it around the body to act as a cryoprotectant. We used flow-through respirometry to measure CO...

Background The purpose was to investigate the effect of the duration of gamete incubation on fertilization rate, embryo cleavage, and embryo quality before and after freezing in mice. MaterialsAndMethods Ovulated oocytes collected from superovulated mice after ip injection of PMSG and hCG were divided randomly into control and experimental groups. Oocytes from the control group were inseminated...