The ability to ship cooled stallion sperm for subsequent freezing at a facility specializing in cryopreservation would be beneficial to the equine industry. Stallion sperm has been centrifuged, cooled to 5 degrees C for 12 h, and frozen without a detrimental effect on motility in a previous study; however, no fertility data were available. Experiment 1 compared the post-thaw motility of sperm c...

A freeze-thaw cycle to -12 degrees C induced several physical and compositional changes in the microsomal membranes isolated from crown tissue of winter wheat (Triticum aestivum L. cv Frederick). Exposing 7-day-old, nonacclimated seedlings to a single freeze-thaw cycle prevented regrowth of the crown and resulted in increased membrane semipermeability. The phospholipid and protein content of mi...

the objective of this study was to evaluate the importance of environmental and genetic factors affecting semen quality; sperm volume (sv), sperm concentration (spco), total sperm (ts), fresh sperm motility (fsm), total fresh motile sperm (tfms), post thaw sperm motility (ptsm), total post thaw motile sperm (tptms), number of produced payout (npp), number of fresh motile sperm in each milt ejac...

Manure fertilization of soil significantly impacts the level of nitrous oxide (N2O) emission. Despite their short duration, periods of significant N2O emissions during soil thaws in winter and spring are an important portion of the total annual emissions from agricultural lands. The goal of this study was to understand the effects of tillage, moisture content and manure application on N2O emiss...

Cryopreservation of spermatozoa plays a significant role in reproductive medicine and fertility preservation. Chicken egg yolk is used as an extender in cryopreservation of human spermatozoa using glycerol egg yolk citrate (GEYC) buffered medium. Even though 50% survival of spermatozoa is generally achieved with this method, the risk of high levels of endotoxins and transmission pathogens from ...

We tested the hypotheses that freezing-induced embolism is related to conduit diameter, and that conifers and angiosperms with conduits of equivalent diameter will exhibit similar losses of hydraulic conductivity in response to freezing. We surveyed the freeze-thaw response of conifers with a broad range of tracheid diameters by subjecting wood segments (root, stem and trunk wood) to a freeze-t...

Semen cryopreservation and artificial insemination (AI) are potentially valuable methods for supporting the breeding management of endangered species like the Asian elephant. Cryopreservation of Asian elephant semen has however proven problematic with respect to maintenance of both adequate semen quality and fertility post-thaw. In this study, nine ejaculates from three adult bulls were used to...

This article presents experimental results and analysis of change in freezing characteristics clays silts with pH moisture content the pore structures. The plastic non-plastic cold regions undergo significant changes thermal properties causing non-equilibrium conditions which can lead to frost-heave, thaw-weakening, thawing-induced landslides, mass wasting events. In geotechnical engineering, p...

Basalt fiber and cement-based materials have been widely applied in engineering structures. In this context, the durability of basalt-fiber-reinforced ordinary silicate mortar was systematically studied under exposure to unilateral salt freezing. The mechanical durability, chloride ion diffusion characteristics, microscopic pore characteristics cement with basalt content levels range 0 kg/m 3 –...

This study aims to characterize and understand the effects of freezing on collagen structures and functionality. Specifically, thermodynamic destabilization of collagen at molecular- and fibril-levels by combination of low temperatures and freezing were experimentally characterized using modulated differential scanning calorimetry. In order to delineate the effects of sub-zero temperature and w...